Organ transplantation is a highly utilized treatment for many medical conditions, yet the number of patients waiting for organs far exceeds the number available. The challenges and limitations currently associated with organ transplantation and technological advances in gene editing techniques have led scientists to pursue alternate solutions to the donor organ shortage. Growing human organs in animals and harvesting those organs for transplantation into humans is one such solution. These chimeric animals usually have certain genes necessary for a specific organ\'s development inhibited at an early developmental stage, followed by the addition of cultured pluripotent human cells to fill that developmental niche. The result is a chimeric animal that contains human organs which are available for transplant into a patient, circumventing some of the limitations currently involved in donor organ transplantation. In this review, we will discuss both the current scientific and legal landscape of human-animal chimera (HAC) research. We present an overview of the technological advances that allow for the creation of HACs, the patents that currently exist on these methods, as well as current public attitude and understanding that can influence HAC research policy. We complement our scientific and public attitude discussion with a regulatory overview of chimera research at both the national and state level, while also contrasting current U.S. legislation with regulations in other countries. Overall, we provide a comprehensive analysis of the legal and scientific barriers to conducting research on HACs for the generation of transplantable human organs, as well as provide recommendations for the future.

Organoids are 3D structures grown from pluripotent stem cells derived from human tissue and serve as in vitro miniature models of human organs. Organoids are expected to revolutionize biomedical research and clinical care. However, organoids are not seen as morally neutral. For instance, tissue donors may perceive enduring personal connections with their organoids, setting higher bars for informed consent and patient participation. Also, several organoid sub-types, e.g., brain organoids and human-animal chimeric organoids, have raised controversy. This systematic review provides an overview of ethical discussions as conducted in the scientific literature on organoids. The review covers both research and clinical applications of organoid technology and discusses the topics informed consent, commercialization, personalized medicine, transplantation, brain organoids, chimeras, and gastruloids. It shows that further ethical research is needed especially on organoid transplantation, to help ensure the responsible development and clinical implementation of this technology in this field.

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Neuronal networks, similar to many other complex systems, self-organize into fascinating emergent states that are not only visually compelling, but also vital for the proper functioning of the brain. Synchronous spatiotemporal patterns, for example, play an important role in neuronal communication and plasticity, and in various cognitive processes. Recent research has shown that the coexistence of coherent and incoherent states, known as chimera states or simply chimeras, is particularly important and characteristic for neuronal systems. Chimeras have also been linked to the Parkinson\'s disease, epileptic seizures, and even to schizophrenia. The emergence of this unique collective behavior is due to diverse factors that characterize neuronal dynamics and the functioning of the brain in general, including neural bumps and unihemispheric slow-wave sleep in some aquatic mammals. Since their discovery, chimera states have attracted ample attention of researchers that work at the interface of physics and life sciences. We here review contemporary research dedicated to chimeras in neuronal networks, focusing on the relevance of different synaptic connections, and on the effects of different network structures and coupling setups. We also cover the emergence of different types of chimera states, we highlight their relevance in other related physical and biological systems, and we outline promising research directions for the future, including possibilities for experimental verification.