背景:心血管疾病是全球最普遍和最主要的死亡原因,这些疾病中最致命和最危险的是心肌梗塞(MI),通常被称为心脏病发作,由于冠状动脉流量不足而发展并导致不可逆的心肌细胞损伤。本研究旨在探讨苦瓜的心脏保护作用,以其抗氧化和抗炎特性而闻名,在大鼠由异丙肾上腺素(ISO)诱导的实验性急性MI模型中。
方法:在研究中,将49只雄性Wistar大鼠分为7组作为对照(CONT),甘油(GLCN),异丙肾上腺素(ISO),500mg/kgMC(MC500),异丙肾上腺素+100mg/kgMC(ISO+MC100),异丙肾上腺素+250毫克/千克MC(ISO+MC250),异丙肾上腺素+500mg/kgMC(ISO+MC500)。对各组施用物质30天。在研究的最后两天(29和30天)通过皮下注射施用异丙肾上腺素(85mg/kg)。在麻醉下最后一次施用物质后24小时进行心电图(ECG)记录和收集动物的血样。血清IL-6、Nrf2、IL-10、HO-1、TNF-α、CK-MB,用ELISA法测定cTn-I和CRP水平。
结果:与ISO组相比,CK-MB的水平,HO-1,TNF-α,CRP,在MC给药组中IL-6和cTn-I在统计学上较低(p<0.05)。此外,MC将ISO诱导的异常ECG变化恢复到正常水平。
结论:结论:心电图检查结果,促炎,抗炎,抗氧化和心脏生物标志物提示MC可能具有心脏保护特性.
BACKGROUND: Cardiovascular diseases are the most prevalent and primary cause of death globally, and the most deadly and dangerous of these diseases is myocardial infarction (MI), commonly known as heart attack, which develops due to insufficient coronary artery flow and causes irreversible myocardial cell damage. This study aimed to investigate the cardioprotective effects of Momordica charantia (MC), known for its antioxidant and anti-inflammatory properties, in an experimental acute MI model induced by isoprenaline (ISO) in rats.
METHODS: In the study, forty-nine male Wistar rats were split up into 7 groups as control (CONT), Glycerin (GLCN), isoprenaline (ISO), 500 mg/kg MC (MC500), isoprenaline+100 mg/kg MC (ISO+MC100), isoprenaline+250 mg/kg MC (ISO+MC250), isoprenaline+500 mg/kg MC (ISO+MC500). Substances were administered to the groups for 30 days. Isoprenaline (85 mg/kg) was administered by subcutaneous injection on the last two days of the study (days of the 29 and 30). Electrocardiogram (ECG) recording and collecting blood samples of the animals were performed 24 hours after the last administration of the substances under the anesthesia. Serum IL-6, Nrf2, IL-10, HO-1, TNF-α, CK-MB, cTn-I and CRP levels were determined by the ELISA method.
RESULTS: Compared to the ISO group, levels of CK-MB, HO-1, TNF-α, CRP, IL-6 and cTn-I were found statistically lower in MC-administered groups (p<0.05). In addition, MC restored ISO-induced abnormal ECG changes to normal levels.
CONCLUSIONS: In conclusion, ECG findings, proinflammatory, anti-inflammatory, antioxidative and cardiac biomarkers suggest that MC may have cardioprotective properties.