Although individual differences in the behavior of animals, sometimes referred to as personality, have recently received considerable attention, the development of such differences remains understudied. We previously found consistent individual differences in behavior in four tests simulating everyday contexts in 74 preweaning age kittens from 16 litters of the domestic cat. To study the development of consistent among-individual differences in four behavioral traits in cats, we followed a subset of these same individuals and repeated the same tests at 6 and 12 months of age. Some individual differences in behavior became increasingly repeatable with age due to a combination of decreased individual-level variance (canalization) and increased among-individual variance; these changes in variance and repeatability continued into adulthood (12 months). We did not observe behavioral syndromes at any age, in contrast to our previous reports in a different population of adult cats. The mechanisms that underlie increased repeatability with age and the possibility of personality structure differing between populations in this species remain to be studied.

Size and shape are important determinants of fitness in most living beings. Accordingly, the capacity of the organism to regulate size and shape during growth, containing the effects of developmental disturbances of different origin, is considered a key feature of the developmental system. In a recent study, through a geometric morphometric analysis on a laboratory-reared sample of the lepidopteran Pieris brassicae, we found evidence of regulatory mechanisms able to restrain size and shape variation, including bilateral fluctuating asymmetry, during larval development. However, the efficacy of the regulatory mechanism under greater environmental variation remains to be explored. Here, based on a field-reared sample of the same species, by adopting identical measurements of size and shape variation, we found that the regulatory mechanisms for containing the effects of developmental disturbances during larval growth in P. brassicae are also effective under more natural environmental conditions. This study may contribute to better characterization of the mechanisms of developmental stability and canalization and their combined effects in the developmental interactions between the organism and its environment.

Spatiotemporal cues orchestrate the development of organs and cellular differentiation in multicellular organisms. For instance, in the root apical meristem an auxin gradient patterns the transition from stem cell maintenance to transit amplification and eventual differentiation. Among the proximal tissues generated by this growth apex, the early, so-called protophloem, is the first tissue to differentiate. This observation has been linked to increased auxin activity in the developing protophloem sieve element cell files as compared to the neighboring tissues. Here we review recent progress in the characterization of the unique mechanism by which auxin canalizes its activity in the developing protophloem and fine-tunes its own transport to guide proper timing of protophloem sieve element differentiation.

Using the centipede model species Strigamia maritima as a subject of study, we illustrate the potential of geometric morphometrics for investigating the development and evolution of segmentation, with a specific focus on post-embryonic segmental patterning. We show how these techniques can contribute detailed descriptive data for comparative purposes, but also precious information on some features of the developmental system that are considered relevant for the evolvability of a segmented body architecture, such as developmental stability and canalization. Morphometric analyses allow to separately investigate several sources of phenotypic variation along a segmented body axis, like constitutive and random segment heteronomy, both within and among individuals. Specifically, in S. maritima, the segmental pattern of ventral sclerite shapes mirrors that of their bilateral fluctuating asymmetry and among-individual variation in associating the most anterior and most posterior segments in diverging from the central ones. Also, among segments, there seems to be a correlation between fluctuating asymmetry and shape variation among individuals, suggesting that canalization and developmental stability are somehow associated. Overall, these associations might stem from a joint influence of the segmental position on the two processes of developmental buffering.