世界正在努力应对2019年冠状病毒病(COVID-19)大流行,其病原体是严重急性呼吸综合征冠状病毒2(SARS-CoV-2)。COVID-19的症状与普通感冒相似,包括发烧,喉咙痛,咳嗽,肌肉和胸痛,脑雾,呼吸困难,嗅觉缺失,Ageusia,和头痛。该疾病的表现可以从无症状到需要住院治疗和通气支持的严重危及生命的疾病。此外,共同关注变种(VOCs)的出现对于大流行的破坏性影响至关重要。这种高度传染性的病毒及其新兴变体挑战了可用的先进病毒诊断方法,以实现高精度测试,并获得更快的结果。这篇评论是为了阐明自然历史,病理学,分子生物学,和COVID-19的有效诊断方法,在收集的样本中检测SARS-CoV-2。我们回顾了用于COVID-19诊断的金标准RT-qPCR方法,以更好地理解和应用于对抗COVID-19大流行。这项全面审查可能会进一步提高对COVID-19大流行管理的认识。
The world is grappling with the coronavirus disease 2019 (COVID-19) pandemic, the causative agent of which is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). COVID-19 symptoms are similar to the common cold, including fever, sore throat, cough, muscle and chest pain, brain fog, dyspnoea, anosmia, ageusia, and headache. The manifestation of the disease can vary from being asymptomatic to severe life-threatening conditions warranting hospitalization and ventilation support. Furthermore, the emergence of mutecated variants of concern (VOCs) is paramount to the devastating effect of the pandemic. This highly contagious virus and its emergent variants challenge the available advanced viral diagnostic methods for high-accuracy testing with faster result yields. This
review is to shed light on the natural history, pathology, molecular biology, and efficient diagnostic methods of COVID-19, detecting SARS-CoV-2 in collected samples. We reviewed the gold standard RT-qPCR method for COVID-19 diagnosis to confer a better understanding and application to combat the COVID-19 pandemic. This comprehensive
review may further develop awareness about the management of the COVID-19 pandemic.