Abstract：
Phage display was first described by George P. Smith when it was shown that virus particles were capable of presenting foreign proteins on their surface. The technology has paved the way for the evolution of various biomolecules presentation and diverse selection strategies. This unique feature has been applied as a versatile platform for numerous applications in drug discovery, protein engineering, diagnostics, and vaccine development. Over the decades, the limits of biomolecules displayed on phage particles have expanded from peptides to proteomes and even alternative scaffolds. This has allowed phage display to be viewed as a versatile display platform to accommodate various biomolecules ranging from small peptides to larger proteomes which has significantly impacted advancements in the biomedical industry. This review will explore the vast array of biomolecules that have been successfully employed in phage display technology in biomedical research.
摘要：
噬菌体展示首先由GeorgeP.Smith描述,当时证明病毒颗粒能够在其表面上呈递外源蛋白。该技术为各种生物分子呈现和多样化选择策略的发展铺平了道路。这一独特的功能已被用作药物发现中众多应用的通用平台，蛋白质工程，诊断,和疫苗开发。几十年来,噬菌体颗粒上展示的生物分子的限制已经从肽扩展到蛋白质组甚至替代支架。这使得噬菌体展示被视为多功能展示平台,以适应从小肽到较大蛋白质组的各种生物分子,这显著影响了生物医学行业的进步。本文将探讨已成功用于生物医学研究中的噬菌体展示技术的大量生物分子。
