■一个主要问题是找到一种有效的生物标志物,能够在存在和不存在风险因素的情况下诊断牙周病。的确,在临床和流行病学调查中,已经描述了吸烟与牙周疾病之间的关联及其对抗菌肽表现的影响。抗菌肽cathelicidin(LL-37)在保持牙周健康方面至关重要。
■这项调查检查并对比了牙周炎吸烟者和非吸烟者唾液中的cathelicidin水平。该研究还试图建立这种生物标志物的诊断能力,以区分牙周健康和疾病。
■该研究涉及从160名参与者中收集未刺激的唾液样本,包括80例被诊断为牙周炎的患者(其中40例为吸烟者,40例为非吸烟者)和80例无牙周炎的患者(40例为吸烟者,40例为非吸烟者)。评估临床牙周参数,包括记录探测袋深度,临床依恋水平,以及探查时出血的百分比。随后,进行酶联免疫吸附测定以定量从上述受试者获得的唾液样品中LL-37的蛋白质水平。
■在非吸烟者牙周炎(NSP)患者中发现唾液LL-37的最高水平,其次是吸烟者牙周炎(SP)组,然后是非吸烟者健康(NSH)组,而在健康吸烟者(HS)组中发现最低水平。同时,LL-37似乎是区分牙周健康和是否存在吸烟的疾病的一个非常好的生物标志物.
牙周炎导致吸烟者和非吸烟者患者的唾液LL-37水平与健康个体相比显著升高。这些水平与牙周参数呈正相关,可以作为预测牙周炎的有价值的诊断工具,而吸烟显著降低了这些水平。
UNASSIGNED: A major issue is finding a valid biomarker able to diagnose periodontal disease with the presence and absence of risk factors. Indeed, the association between smoking and periodontal diseases and its impact on the manifestation of antimicrobial peptides has been delineated in clinical and epidemiological investigations. The antimicrobial peptide cathelicidin (LL-37) is pivotal in preserving periodontal health.
UNASSIGNED: This investigation examines and contrasts the levels of cathelicidin in the saliva of smokers and nonsmokers of periodontitis. The study also seeks to establish this biomarker\'s diagnostic ability to differentiate between periodontal health and disease.
UNASSIGNED: The study involved the collection of unstimulated saliva samples from 160 participants, comprising 80 patients diagnosed with periodontitis (40 of whom were smokers and 40 were nonsmokers) and 80 periodontitis-free individuals (40 smokers and 40 nonsmokers). The clinical periodontal parameters were assessed, including recording the probing pocket depth, the level of clinical attachment, and the percentage of bleeding on probing. Subsequently, enzyme-linked immunosorbent assays were conducted to quantify the protein levels of LL-37 in the saliva samples obtained from the subjects mentioned above.
UNASSIGNED: The highest level of salivary LL-37 was found in the nonsmoker periodontitis (NSP) patients, followed by the group of smoker periodontitis (SP) and then nonsmoker healthy (NSH) group, while the lowest level was found in the healthy smoker (HS) group. At the same time, the LL-37 seems to be a very good biomarker in differentiating periodontal health from disease with the presence and absence of smoking.
UNASSIGNED: Periodontitis results in a significant elevation of salivary LL-37 levels in smoker and nonsmoker patients compared to healthy individuals. These levels are positively correlated with the periodontal parameter and can serve as a valuable diagnostic tool to predict periodontitis, whereas smoking significantly reduces these levels.