顺铂等化疗药物对癌症治疗有效,但仍无特异性,易受耐药性的影响,并对器官系统产生多种副作用。沸石咪唑酯骨架-8,一种MOF,引起了人们的注意,包括作为靶向癌症治疗的药物递送方法。在这项研究中,ZIF-8/二氧化硅纳米复合材料是使用Stober技术使用一锅法水热技术合成的。我们研究了植物合成的GPt和化疗药物顺铂CPt对ZIF-8/Silica癌症治疗靶向疗效的影响。纹理,形态学,采用XRD等不同表征技术对ZIF-8/二氧化硅上Pt的化学环境进行了分析,FT-IR,BET,漫反射光谱,SEM-EDX,TEM,zeta电位,和TGA分析。CPt和GPt吸附的等温行为使用等温线模型如Langmuir,Freundlich,和Temkin等温线.表明GPt和CPt吸附效率的吸附动力学受Pt络合物浓度和ZIF-8/二氧化硅吸附位点的影响。在ZIF-8/二氧化硅上,GPt(86%和4.3%)和CPt(91%和4.5%)的高包封效率和负载能力是明显的。使用透析膜技术,纳米复合材料显示出pH敏感的Pt释放。例如,在72小时内pH=6.6下观察到GPt的高释放(93%),而在pH7.4在72小时内释放降低至50%。使用MCF7(乳腺癌细胞)和HFF-1(人包皮成纤维细胞)细胞在体外研究了纳米制剂的抗癌活性。研究结果表明,GPt与CPt一样有效;用ZIF-8/二氧化硅/Cp/PEG处理的MCF7细胞的EC50值为94.86µg/mL,而对于ZIF-8/二氧化硅/GPt/PEG,则为60.19µg/mL。
A chemo-drug such as cisplatin is effective for cancer treatment but remains non-specific, is susceptible to drug resistance, and induces several side effects on organ systems. Zeolitic imidazolate framework-8, a type of MOF, has gained attention, including as a drug delivery method for targeted cancer therapeutics. In this
study, ZIF-8/Silica nanocomposite was synthesized using a one-pot hydrothermal technique using the Stober technique. We studied the effect of phyto-synthesized GPt and chemo-drug cisplatin CPt on ZIF-8/Silica for targeted efficacy of cancer therapy. The texture, morphology, and chemical environment of Pt on ZIF-8/Silica were analyzed using different characterization techniques such as XRD, FT-IR, BET, diffuse reflectance spectroscopy, SEM-EDX, TEM, zeta potential, and TGA analysis. The isothermal behavior of CPt and GPt adsorption was investigated using isotherm models like Langmuir, Freundlich, and Temkin isotherm. The adsorption kinetics indicating the adsorption efficiency of GPt and CPt are influenced by the concentration of Pt complex and the adsorption sites of ZIF-8/Silica. A high entrapment efficiency and loading capacity of GPt (86% and 4.3%) and CPt (91% and 4.5%) were evident on ZIF-8/Silica. The nanocomposite showed a pH-sensitive Pt release using a dialysis membrane technique. For instance, a high release of GPt (93%) was observed under pH = 6.6 in 72 h, while the release reduced to 50% at pH 7.4 in 72 h. The anti-cancer activity of nanoformulations was studied in vitro using MCF7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) cells. The findings demonstrated that GPt is as effective as CPt; the EC50 value for MCF7 cells treated with ZIF-8/Silica/Cp/PEG was 94.86 µg/mL, whereas for ZIF-8/Silica/GPt/PEG it was 60.19 µg/mL.