背景:先前的几项研究已经确定了肠道微生物组在儿童自闭症谱系障碍(ASD)中的潜在作用,但对病毒的变化可能与ASD有关的情况知之甚少。我们旨在了解ASD儿童肠道DNA病毒的变化。
方法:采用病例对照研究,在考虑年龄的同时观察到13个两个孩子的家庭,出生模式,抗生素使用史,和疫苗接种史,以尽量减少混杂因素的影响。对11名ASD儿童和12名健康非ASD儿童的粪便样本成功进行了DNA病毒宏基因组测序。检测并分析了参与者粪便DNA病毒的基本组成和基因功能。最后,比较了ASD儿童及其健康兄弟姐妹的DNA病毒的丰度和多样性。
结果:发现3-11岁儿童的肠道DNA病毒主要由Caudovirales的Siphoviridae家族控制。DNA基因编码的蛋白质主要执行遗传信息传递和代谢的功能。比较ASD和健康非ASD儿童的肠道DNA病毒,Caudovirales和Petitvirales的丰度均呈显著负相关(r=-0.902,P<0.01),在顺序和家庭水平上,病毒的相对丰度没有统计学上的显着差异,以及Skunavirus属水平的相对丰度差异(Z=-2.157,P=0.031)。ASD儿童的病毒α多样性减少,但各组之间的α多样性和β多样性没有统计学差异。
结论:这项研究表明,在ASD儿童的肠道DNA毒力组中,Skunavirus丰度升高,α多样性降低,但α和β多样性变化无统计学差异。这提供了有关微生物组和ASD之间关系的病毒学方面的初步累积信息,并且应该有利于未来的多组学和大样本研究ASD儿童的肠道微生物。
Several previous studies have identified a potential role that the gut microbiome can play in autism spectrum disorder (ASD) in children, but little is known about how variations in the
virome may be involved in ASD. We aimed to understand the changes in the gut DNA
virome of children with ASD.
A
case-control study was presented, in which 13 two-children families were observed while considering the age, mode of birth, history of antibiotic use, and vaccination history to minimize the influence of confounding factors. DNA viral metagenomic sequencing was successfully performed on stool samples from 11 children with ASD and 12 healthy non-ASD children. The basic composition and gene function of the participants\' fecal DNA virome were detected and analyzed. Finally, the abundance and diversity of the DNA
virome of children with ASD and their healthy siblings were compared.
The gut DNA virome in children aged 3-11 years was found to be dominated by the Siphoviridae family of Caudovirales. The proteins encoded by the DNA genes mainly carry out the functions of genetic information transmission and metabolism. Compared the gut DNA
virome of ASD and healthy non-ASD children, their abundance of Caudovirales and Petitvirales both showed a significant negative correlation (r = -0.902, P < 0.01), there was no statistically significant difference in the relative abundance of viruses at the order and family levels, and a difference in the relative abundance at the genus level for Skunavirus (Ζ = -2.157, P = 0.031). Viral α diversity was reduced in children with ASD, but α diversity and β diversity did not differ statistically between groups.
This study indicates that elevated Skunavirus abundance and decreased α diversity in the gut DNA virulence group of children with ASD, but no statistically significant difference in the change in alpha and beta diversity. This provides preliminary cumulative information on virological aspects of the relationship between the microbiome and ASD, and should benefit future multi-omics and large sample studies on the gut microbes in children with ASD.