背景:蚕豆(ViciafabaL.)是近东最早的驯化作物之一。今天,这种豆类是全球重要的蛋白质饲料和食品,并继续在整个中东的烹饪传统中发挥重要作用。地中海地区,中国和埃塞俄比亚。适应广泛的土壤类型,蚕豆育种的主要目标是提高产量,对生物和非生物胁迫的抗性,种子质量和其他农艺性状。旨在增强蚕豆育种计划的基因组方法需要高质量的遗传连锁图谱,以促进定量性状基因座分析和基因标记,以用于标记辅助选择。这项研究的目的是通过将迄今为止在该作物中报告的最相关地图中的信息结合起来,在蚕豆中构建参考共识图。
结果:两种方法的组合,增加不同作图群体中锚定位点的数量,并加入相应的遗传图谱,用于在蚕豆中开发参考共识图。该图谱是从四个亲本系衍生的三个主要重组近交种群中构建的,包含729个标记,基于69个常见基因座。它跨越4,602cM,在六个主要连锁群或染色体中的323到1041个基因座,和每6cM一个基因座的平均标记密度。在共识图和各个图之间通常很好地保持了轨迹顺序。
结论:我们构建了一个可靠且相当密集的共识遗传连锁图谱,该图谱将作为蚕豆研究和育种中基因组方法的基础。核心地图包含的标记数量比以前的任何单个地图都多,覆盖现有的差距,并实现了更广泛的覆盖大型蚕豆基因组作为一个整体。该工具可作为不同遗传背景研究的参考资源,并提供了使用不同标记技术时转移遗传信息的框架。结合同势方法,共识图将增加选定基因组区域的标记密度,并将有助于未来的蚕豆分子育种应用。
BACKGROUND: Faba bean (Vicia faba L.) is among the earliest domesticated crops from the Near East. Today this legume is a key protein feed and food worldwide and continues to serve an important role in culinary traditions throughout Middle East, Mediterranean region, China and Ethiopia. Adapted to a wide range of soil types, the main faba bean breeding objectives are to improve yield, resistance to biotic and abiotic stresses, seed quality and other agronomic traits. Genomic approaches aimed at enhancing faba bean breeding programs require high-quality genetic linkage maps to facilitate quantitative trait locus analysis and gene tagging for use in a marker-assisted selection. The objective of this study was to construct a reference
consensus map in faba bean by joining the information from the most relevant maps reported so far in this crop.
RESULTS: A combination of two approaches, increasing the number of anchor loci in diverse mapping populations and joining the corresponding genetic maps, was used to develop a reference
consensus map in faba bean. The map was constructed from three main recombinant inbreed populations derived from four parental lines, incorporates 729 markers and is based on 69 common loci. It spans 4,602 cM with a range from 323 to 1041 loci in six main linkage groups or chromosomes, and an average marker density of one locus every 6 cM. Locus order is generally well maintained between the
consensus map and the individual maps.
CONCLUSIONS: We have constructed a reliable and fairly dense
consensus genetic linkage map that will serve as a basis for genomic approaches in faba bean research and breeding. The core map contains a larger number of markers than any previous individual map, covers existing gaps and achieves a wider coverage of the large faba bean genome as a whole. This tool can be used as a reference resource for studies in different genetic backgrounds, and provides a framework for transferring genetic information when using different marker technologies. Combined with syntenic approaches, the
consensus map will increase marker density in selected genomic regions and will be useful for future faba bean molecular breeding applications.