目的:研究印度北部一家三级医院的厌氧菌感染谱和各种感染中临床相关厌氧菌的发生频率。
方法:在这项为期两年(2018-2019年)的回顾性研究中,共有22,177份样本进行了厌氧和需氧处理。样本包括血液,脓液,体液,坏死组织,脓肿,腹膜液,和其他易患厌氧菌感染的条件标本。通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOFMS)鉴定回收的细菌分离株。
结果:在经过厌氧处理的22,177个样本中,1094显示出显着的厌氧生长,产生1464种厌氧菌。脆弱拟杆菌(12.7%)和后亲肽(12.2%)是最主要的厌氧菌,而大肠杆菌(32.6%)和金黄色葡萄球菌(13.2%)是多微生物生长中最常见的兼性厌氧菌。673/1094样品的需氧和厌氧数据均可用,其中68%为多微生物病因,32%为单抗微生物。在可获得完整临床信息的病例中(504/1094),大多数厌氧菌从皮肤和软组织感染(36.3%)和腹腔内感染(17.1%)中恢复.最常见厌氧菌生长的临床标本是脓肿(29.1%),其次是糖尿病足溃疡(14.1%)。
结论:该研究记录了各种感染中具有临床意义的厌氧菌的频率,以及它们与微生物生长中需氧菌的关系。本研究可以帮助我们设计更好的治疗策略来对抗厌氧菌和厌氧菌在厌氧菌感染。这通常是经验性的。此外,这些数据可以使临床医生了解厌氧菌感染模式的变化,这仍然是一个被忽视的问题。
OBJECTIVE: To
study the spectrum of anaerobic infections and the frequency of clinically relevant anaerobes in various infections in a tertiary care hospital in North India.
METHODS: In this two-year (2018-2019) retrospective
study, a total of 22,177 samples were processed both anaerobically and aerobically. The samples included blood, pus, body fluids, necrotic tissues, abscess, peritoneal fluids, and other specimens of conditions predisposing to anaerobic infections. The recovered bacterial isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).
RESULTS: Of the 22,177 samples processed anaerobically, 1094 showed significant anaerobic growth yielding 1464 anaerobes. Bacteroides fragilis (12.7%) and Peptoniphilus harei (12.2%) were the most predominant anaerobes, whereas Escherichia coli (32.6%) and Staphylococcus aureus (13.2%) were the most frequently recovered facultative anaerobes in polymicrobial growth. Both aerobic and anaerobic data were available for 673/1094 samples, of which 68% represented a polymicrobial etiology and 32% as monomicrobial. Of the cases where complete clinical information was available (504/1094), the majority of the anaerobes were recovered from the skin and soft tissue infections (36.3%) and intra-abdominal infections (17.1%). The clinical specimen most frequently growing anaerobes was abscess (29.1%), followed by diabetic foot ulcers (14.1%).
CONCLUSIONS: The
study documents the frequency of clinically significant anaerobic bacteria in various infections, and their associations with aerobes in polymicrobial growth. The present
study may aid us in devising better therapeutic strategies against both aerobes and anaerobes in anaerobic infections, which is often empirical. Besides, the data can update clinicians with the changing patterns of anaerobic infections, which remains a neglected concern.