目的:本研究旨在通过系统评价和荟萃分析,根据年龄和种族,研究口腔中这些细菌计数受复发性口疮性口炎(RAS)状态的调节。
方法:在英文文献数据库中搜索相关的病例对照研究,韩语,和中文,直到2020年6月使用关键字,并对文献进行筛选和整理,以进行ReviewManager分析。对纳入文献进行敏感性分析和质量检查。
结果:从选择过程中,8项研究通过聚合酶链反应(PCR)和4项研究通过下一代测序测量口腔细菌计数.健康控制,RAS的溃疡期(UC-RAS),非溃疡期RAS(Non-UC-RAS)组包括442,473和386名参与者,共12项研究.对于PCR检测,健康对照组和RAS组Veillonella和链球菌计数的平均差异(95%置信区间)分别为-1.91(-2.41~-1.41)和-1.34(-1.85~-0.83)(P<0.0001)。“下一代”测序(NGS)和PCR方法的细菌计数结果相似。UC-RAS组的Veillonella和链球菌计数显著低于非UC-RAS组(P<0.0001)。RAS和年龄≥30岁的对照组之间的Veillonella和链球菌计数差异大于年龄<30岁的对照组。在物种层面,RAS的患病率与Veillonelladispar计数呈负相关。
结论:Veillonella和链球菌的计数与RAS的恢复和进展密切相关,尤其是中年患者。在治疗RAS时应考虑调整口腔微生物群。
OBJECTIVE: This study aimed to investigate that these bacteria counts in the oral cavity were modulated by the recurrent aphthous stomatitis (RAS) status according to age and ethnicity with a systematic
review and meta-analysis.
METHODS: The relevant case-control studies were searched in the literature database in English, Korean, and Chinese until June 2020 using keywords, and the literature was screened and collated for
Review Manager analysis. Sensitivity analysis and quality check of the included literature were conducted.
RESULTS: From the selection process, oral bacteria counts were measured by polymerase chain reaction (PCR) in 8 studies and next-generation sequencing in 4 studies. Healthy control, ulcerative phases of RAS (UC-RAS), non-ulcerative phases of RAS (Non-UC-RAS) groups included 442, 473, and 386 participants in a total of 12 studies. For PCR detection, mean differences (95 % confidence intervals) of
Veillonella and Streptococcus counts between the healthy-control and RAS groups were -1.91 (-2.41 ∼ -1.41) and -1.34 (-1.85 ∼ -0.83)(P < 0.0001). The bacteria count results by \"Next-generation\" sequencing (NGS) and PCR methods were similar. Significantly lower Veillonella and Streptococcus counts were observed in the UC-RAS group than in the non-UC-RAS group (P < 0.0001).
Veillonella and Streptococcus count differences between RAS and controls aged ≥30 years were greater than those aged <30 years. At the species level, the prevalence of RAS had a negative relation with
Veillonella dispar count.
CONCLUSIONS: Counts of Veillonella and Streptococcus are strongly correlated with the recovery and progression of RAS, especially in middle-aged patients. Adjustment of oral microbiota should be considered in the treatment of RAS.