慢性压力被认为是导致各种疾病的严重危险因素,包括焦虑和认知能力下降。本研究旨在探讨牛至提取物改善慢性不可预知应激(CUS)引起的焦虑样行为和学习记忆缺陷的作用。
对雄性大鼠执行了10天的CUS方案,在第10天,他们的焦虑,学习,和记忆状态进行了评估。之后,除了CUS,用牛至提取物治疗大鼠2周。然后,BDNF的表达,TrkB,并对大鼠海马和前额叶皮质TLR2/4基因进行评价。此外,肝脏和肾脏相关的血清参数,包括甘油三酯,总胆固醇,HDL,LDL,肌酐,尿素,血清葡萄糖,丙氨酸氨基转移酶,和天冬氨酸转氨酶进行评估。Further,研究了提取物的致死作用及其对动物体重的影响。
行为测试证实了CUS引起的焦虑样行为和学习记忆功能损害。相比之下,提取物的给药可以显著缓解精神缺陷和减少焦虑样行为。分子评估表明,CUS可以显着降低BDNF和TrkB基因的表达水平,同时增加TLR2和TLR4的表达水平。相比之下,在提取物处理的动物中,BDNF和TrkB的mRNA水平显著增加,然而TLR2和TLR4mRNA水平降低。此外,提取物的消耗导致体重增加,同时对肝脏和肾脏功能没有杀伤力和有害影响。
这些发现表明提取物的抗焦虑性质及其对认知功能障碍的改善作用。
Chronic stress is considered a severe risk factor leading to various disorders, including anxiety and cognitive decline. The present
study aimed to investigate the effects of Origanum vulgare (oregano) extract on improving anxiety-like behavior and learning and memory defection caused by chronic unpredictable stress (CUS).
A 10-day CUS protocol was executed on male rats, and on day 10, their anxiety, learning, and memory status were evaluated. After that, in addition to the CUS, the rats were treated with the oregano extract for 2 weeks. Then, the expression of BDNF, TrkB, and TLR2/4 genes in the hippocampus and prefrontal cortex of the rats was evaluated. Also, the liver- and kidney-related serum parameters, including triglycerides, total cholesterol, HDL, LDL, creatinine, urea, serum glucose, alanine aminotransferase, and aspartate aminotransferase were assessed. Further, the extract\'s lethal effect and its impact on animals\' body weight were investigated.
Behavioral tests confirmed the anxiety-like behavior and learning-memory function impairment caused by CUS. In contrast, the administration of the extract could significantly alleviate the mental deficiencies and diminished anxiety-like behaviors. Molecular assessments showed that CUS could markedly decrease the BDNF and TrkB genes\' expression levels while increasing that of TLR2 and TLR4. In contrast, in extract-treated animals, mRNA levels of BDNF and TrkB considerably increased, yet TLR2 and TLR4 mRNA levels reduced. Additionally, consumption of the extract caused weight gain, while having no lethality and detrimental effect on the liver and kidneys functions.
These findings indicate the anxiolytic properties of the extract and its improving effect on cognitive dysfunction.