背景:已经对Toll样受体(TLR)基因的rs3775290C/T和rs3853839C/G单核苷酸多态性与手之间的关系进行了一些病例对照研究,脚,和口蹄疫(HFMD)的易感性和严重程度。这项荟萃分析旨在对中国汉族人群中与TLR3基因的C/T(rs3775290)多态性或TLR7基因的C/G(rs3853839)多态性相关的HFMD易感性和严重程度进行系统综述。
方法:使用PubMed进行了计算机搜索,WebofScience,Embase,CNKI,CBM,VIP,和万方数据库。时间范围是从数据库建立到2021年7月30日。根据纳入和排除标准选择的文章进行数据提取和方法学质量评价。采用RevMan5.4和Stata16.0进行荟萃分析,并计算合并比值比(OR)值和95%置信区间(CIs).进行敏感性和发表偏倚评估。
结果:选择了8篇文章和9项研究。其中,TLR3rs3775290研究中有858例和577例对照,TLR7rs3853839研究中有2151例和1554例对照.关于TLR3的rs3775290,重度T型个体的易感性大于C型个体[OR=1.34,95CI(1.10,1.64),P=.004]。严重TT基因型个体的易感性是CC基因型个体的1.61倍[95CI(1.07,2.43),P=0.02],而对手足口病的易感性不受基因型的影响。关于TLR7基因的rs3853839,C等位基因携带者比G等位基因携带者具有更高的发生HFMD的风险。CC+CG个体对HFMD的易感性是GG个体的1.24倍[95CI(1.07,1.43),P=.004]。然而,未发现这种多态性与重度类型的严重程度之间存在关系.在这项研究中没有观察到显著的发表偏倚。
结论:TLR3的rs3775290(C/T)与严重类型的易感性有关,而TLR7的rs3853839(C/G)与HFMD易感性相关。然而,由于研究的数量和质量有限,上述结论尚未得到更多高质量研究的证明。
BACKGROUND: Several
case-control studies have been conducted on the relationship between rs3775290 C/T and rs3853839 C/G single nucleotide polymorphisms of the Toll-like receptor (TLR) gene and hand, foot, and mouth disease (HFMD) susceptibility and severity. This meta-analysis aimed to offer a systemic review of HFMD susceptibility and severity among the Chinese Han population associated with the C/T (rs3775290) polymorphism of the TLR3 gene or C/G (rs3853839) polymorphism of the TLR7 gene.
METHODS: A computer search was conducted using PubMed, Web of Science, Embase, CNKI, CBM, VIP, and WanFang databases. The time ranges were from database establishment to 30/7/2021. Articles selected according to the inclusion and exclusion criteria underwent data extraction and methodological quality evaluation. RevMan 5.4 and Stata 16.0 were adopted for meta-analysis, and the incorporated odds ratio (OR) values and 95% confidence intervals (CIs) were calculated. Sensitivity and publication bias assessments were performed.
RESULTS: 8 articles with 9 studies were selected. Among them, there were 858 cases and 577 controls in TLR3 rs3775290 studies as well as 2151 cases and 1554 controls in TLR7 rs3853839 studies. Regarding rs3775290 of TLR3, susceptibilities of the severe type of T-possessing individuals were larger than those of C-possessing individuals [OR = 1.34, 95%CI (1.10, 1.64), P = .004]. The susceptibility of individuals with the severe TT genotype was 1.61 times that of individuals with the CC genotype [95%CI (1.07, 2.43), P=0.02], while susceptibility to HFMD was not influenced by the genotype. In terms of the rs3853839 of the TLR7 gene, C allele carriers have a higher risk of developing HFMD than G allele carriers. The susceptibility to HFMD in CC+CG individuals was 1.24 times than that in GG individuals [95%CI (1.07, 1.43), P = .004]. However, no relationship was found between this polymorphism and severity of the severe type. No significant publication bias was observed in this study.
CONCLUSIONS: rs3775290 (C/T) of TLR3 is associated with susceptibility to the severe type, whereas rs3853839 (C/G) of TLR7 is associated with susceptibility to HFMD. However, owing to the limited quantity and quality of the research, the aforementioned conclusions are yet to be justified by more high-quality research.