Abstract：
Toll-like receptor 3 (TLR3) and TLR7 genes are involved in the host immune response against viral infections including SARS-COV-2. This study aimed to investigate the association between the TLR3(rs3775290) and TLR7(rs179008) polymorphisms with the prognosis and susceptibility to COVID-19 pneumonia accompanying SARS-COV-2 infection. This case-control study included 236 individuals: 136 COVID-19 pneumonia patients and 100 age and sex-matched controls. Two polymorphisms (TLR3 rs3775290 and TLR7 rs179008) were genotyped by allelic discrimination through TaqMan real-time PCR. This study also investigated predictors of mortality in COVID-19 pneumonia through logistic regression. The mutant \'T/T\' genotypes and the \'T\' alleles of TLR3(rs3775290) and TLR7(rs179008) polymorphisms were significantly associated with increased risk of COVID-19 pneumonia. This study did not report association between the mutant \'T/T\' genotypes of TLR3(rs3775290) and TLR7(rs179008) and the disease outcome. In multivariate analysis, the independent predictors of mortality in COVID-19 pneumonia were male sex, SPO2 ≤ 82%, INR > 1, LDH ≥ 1000 U/l, and lymphocyte count<900/mm3 (P < 0.05).
摘要：
Toll样受体3(TLR3)和TLR7基因参与宿主针对包括SARS-COV-2的病毒感染的免疫应答。本研究旨在探讨TLR3(rs3775290)和TLR7(rs179008)多态性与SARS-COV-2感染并发COVID-19肺炎的预后和易感性的关系。这项病例对照研究包括236名个体：136名COVID-19肺炎患者和100名年龄和性别匹配的对照。通过TaqMan实时PCR，通过等位基因区分对两个多态性（TLR3rs3775290和TLR7rs179008）进行基因分型。这项研究还通过逻辑回归分析了COVID-19肺炎死亡率的预测因素。TLR3(rs3775290)和TLR7(rs179008)多态性的突变T/T基因型和T等位基因与COVID-19肺炎风险增加显著相关。本研究未报道TLR3(rs3775290)和TLR7(rs179008)的突变T/T基因型与疾病转归之间的关联。在多变量分析中，COVID-19肺炎死亡率的独立预测因子是男性，SPO2≤82%,INR>1,LDH≥1000U/l,淋巴细胞计数<900/mm3(P<0.05)。
