液相微萃取(LPME)具有从不同样品基质中分离有机物质的巨大潜力。在这项工作中,首次应用LPME研究了二苯哌啶醇在不同生物流体中的生物分布。器官,和使用致命中毒病例的大脑区域。由于一直没有关于联苯的LPME的报道,支撑液膜(SLM)的影响,受体和供体相,首先考察了提取时间对LPME性能的影响。发现2-壬酮和2-硝基苯基辛基醚(NPOE)的溶剂对于来自生物流体和组织样品的二苯idol的LPME是稳定且有效的SLM,分别。在稳定状态下,不同样品基质的LPME回收率在87%-91%范围内。由于LPME的清除能力以及致命中毒病例中的二苯偶醇浓度相对较高,所提出的LPME系统通过使用HPLC-UV测定的相关样品基质进行验证。方法显示良好的线性(R²≥0.9943),检测限分别为0.30mgL-1、0.28mgL-1和2.7μg-1,尿液,还有肝脏样本,分别。同时,精度(≤13%),准确度(90-110%),基质效应(±15%)在较低的情况下令人满意,中等,和高浓度。此外,稳定性,结转,稀释完整性满足ASB标准036的要求。最后,所提出的方法已成功地应用于评估二苯乙多在五种不同生物流体中的生物分布,五个器官,和一个致命中毒病例的六个大脑区域。一般来说,二苯乙多在生物流体中的分布低于在器官和大脑区域的分布,在肝脏中观察到最高浓度的二苯哌啶醇,这对于法医毒物分析中检验样品的选择非常重要。因此,LPME被证明是法医学领域生物分布和死后再分布调查的有力工具。
Liquid-phase microextraction (LPME) possesses a high potential to isolate organic substances from different sample matrices. In this work, LPME was applied for the first time to investigate the biodistribution of diphenidol in different biofluids, organs, and brain regions using a fatal poisoning
case. Since the LPME of diphenidol hasn\'t been reported, the effect of supported liquid membrane (SLM), acceptor and donor phases, and extraction time on LPME performance was investigated first. The solvents of 2-nonanone and 2-nitrophenyl octyl ether (NPOE) were found to be stable and efficient SLMs for LPME of diphenidol from biofluids and tissue samples, respectively. At steady state, the LPME recoveries for different sample matrices were in the range of 87 %-91 %. Due to the clean-up capability of LPME and the relatively high concentration of diphenidol in the fatal poisoning
case, the proposed LPME systems were validated with related sample matrices using HPLC-UV for the determination. The methods displayed good linearity (R² ≥ 0.9943), and the limits of detection were 0.30 mg L-1, 0.28 mg L-1, and 2.7 μg g-1 for blood, urine, and liver samples, respectively. Meanwhile, the precision (≤13%), accuracy (90-110%), and matrices effect (±15%) were satisfactory at low, medium, and high concentrations. In addition, the stability, carryover, and dilution integrity met the requirements of ASB Standard 036. Finally, the proposed method was successfully applied to evaluate the biodistribution of diphenidol in five different biofluids, five organs, and six brain regions from a fatal poisoning
case. Generally, the distribution of diphenidol in biofluids was lower than that in the organs and brain regions, and the highest concentration of diphenidol was observed in the liver, which is very important for the selection of inspection samples in forensic toxicological analysis. Therefore, LPME was proved to be a powerful tool for the investigation of biodistribution and postmortem redistribution in the fields of forensics.