肿瘤坏死因子(TNF)是一种重要的细胞因子,可以通过结合肿瘤坏死因子受体(TNFR)来调节多种细胞反应。我们研究了中华绒螯蟹的TNF是否可以调节血细胞的增殖。结果表明,EsTNF和EsTNFR在所有受试组织中均呈组成型表达,包括心脏,肝胰腺,肌肉,ill,胃,肠子,和血细胞。我们发现血细胞中存在低水平的EsTNF和EsTNFR转录物。受金黄色葡萄球菌或副溶血性弧菌刺激后,血细胞中的基因表达水平显着增加。我们还发现一些与细胞增殖相关的基因在脉冲rEsTNF刺激的血细胞中表达水平高于对照组。我们还用RNAi技术击倒了EsTNFR基因。结果表明,当TNF不结合TNFR时,与对照组相比,这些与细胞增殖相关的基因的表达水平明显下调。我们使用Edu技术重复上述实验,结果相似。与对照组相比,rEsTNF刺激的血细胞表现出更显著的增殖,敲除EsTNFR基因后增殖率显著下调。因此,我们表明,TNF结合TNFR可以影响E.sinensis血细胞的增殖,这可能表现为影响一些增殖相关基因的表达。
Tumor necrosis factor (TNF) is an important cytokine that can regulate a variety of cellular responses by binding tumor necrosis factor receptor (
TNFR). We studied whether the TNF of Eriocheir sinensis can regulate hemocyte proliferation. The results showed that the EsTNF and EsTNFR were constitutively expressed in all tested tissues, including the heart, hepatopancreas, muscles, gills, stomachs, intestines, and hemocytes. We found that low levels of EsTNF and EsTNFR transcripts were present in hemocytes. The gene expression levels were significantly increased in the hemocytes after being stimulated by Staphylococcus aureus or Vibrio parahaemolyticus. We also found some genes related to cell proliferation were expressed at a higher level in pulsing rTNF-stimulated hemocytes compared with the control group. We also knocked down the EsTNFR gene with RNAi technology. The results showed that the expression level of these genes related to cell proliferation was significantly down-regulated compared with the control group when the TNF does not bind
TNFR. We used Edu technology to repeat the above experiments and the results were similar. Compared with the control group, the hemocytes stimulated by rTNF showed more significant proliferation, and the proliferation rate was significantly down-regulated after knocking down the EsTNFR gene. Therefore, we indicate that TNF binding
TNFR can affect the proliferation of E. sinensis hemocytes, which might be manifested by affecting the expression of some proliferation-related genes.