背景:结核脊柱炎约占所有骨骼结核病例的50%。维生素D在免疫系统中起作用。维生素D有助于TLR-2和TLR-4的激活,它们在结核感染过程中发挥作用。本研究的目的是研究口服补充维生素D对结核性脊柱炎患者TLR-2和TLR-4水平的影响。
方法:本研究使用真实的实验设计,对照组的前测试后测试(对照组的前测试后测试)。通过ELISA测量TLR-2和TLR-4。重复方差分析,方差分析测试,在SPSS程序上使用Kolmogorov-Smirnov正态检验对结果进行统计分析。
结果:在10,000IU和5000IU的剂量组中,维生素D水平显著增加,在第4周和第8周观察到TLR-2和TLR-4(p<0.05)。在对照组中,没有显着增加。
结论:维生素D补充剂可显著提高TLR-2和TLR-4水平。补充维生素D10,000IU/天持续8周可以增加维生素D水平>50ng/dl,以最佳地充当免疫调节剂。
BACKGROUND: Tuberculosis spondylitis accounts for approximately 50% of all cases of skeletal tuberculosis. Vitamin D plays a role in the immune system. Vitamin D helps in the activation of TLR-2 and TLR-4, which play a role in the process of tuberculosis infection. The objective of this
study was to investigate the effect of oral supplementation with vitamin D on TLR-2 and TLR-4 levels in tuberculosis spondylitis patients.
METHODS: The true Experiment Design Pretest-Posttest with Control Group (Pretest-Posttest with Control Group) was used for this research. TLR-2 and TLR-4 were measured by ELISA. Repeated ANOVA, ANOVA tests, and Kolmogorov-Smirnov normality tests on the SPSS program were used to statistically analyze the results.
RESULTS: In the dose groups of 10,000 IU and 5000 IU, significant increases in the levels of vitamin D, TLR-2, and TLR-4 were observed at weeks 4 and 8 (p < 0.05). In the control group, there was no significant increase.
CONCLUSIONS: Vitamin D supplements can significantly increase TLR-2 and TLR-4 levels. Supplementation with vitamin D 10,000 IU/day for 8 weeks can increase vitamin D levels > 50 ng/dl to optimally act as an immunomodulator.