我们旨在通过体内和体外实验，探讨不同辐射剂量的60Co-γ辐照人参不定根（GAR）对其提取物（GARSE）降血糖作用的影响。5kGy照射后，GARSE总皂苷增加了4.50%，2,2-二苯基-1-吡啶酰肼(DPPH)自由基清除能力提高了5.10%。在50μg/mL时，5kGy照射的GARSE对高糖损伤的人肾小球系膜细胞（HMC）表现出优异的保护作用。用500mg/kg·BW的5kGy照射GARSE喂养1型糖尿病（T1DM）小鼠4周后，与未照射的相比，葡萄糖值降低了16.0％。Keap1/Nrf2/HO-1通路被激活，氧化应激减弱，进一步缓解了T1DM。
We aimed to explore the effects of the 60Co-γ irradiated ginseng adventitious root (GAR) with different radiation doses on the hypoglycemic effects of its extract (GARSE) through in vivo and in vitro experiments. The total saponin of GARSE was increased by 4.50% after 5 kGy irradiation, and the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability was enhanced by 5.10%. At 50 μg/mL, GARSE irradiated by 5 kGy displayed superior protective effects on human glomerular mesangial cells (HMCs) with high glucose damage. After feeding type 1 diabetes mellitus (T1DM) mice with GARSE irradiated by 5 kGy at 500 mg/kg·BW for 4 weeks, the glucose values was decreased by 16.0% compared with the unirradiated. The Keap1/Nrf2/HO-1 pathway was activated and the oxidative stress was attenuated, which further alleviated T1DM.
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