尖孢镰刀菌(F.尖孢)是干鱼的常见污染物,这种生物在干鱼产品中合成的T-2会带来严重的公共卫生风险。在这项研究中,我们研究了iturinA的作用,由枯草芽孢杆菌产生的环状脂肽,关于尖孢酵母T-2毒素的生长和合成,并进行了转录组学。结果表明,随着iturinA浓度的增加,iturinA对尖孢酵母的抑制作用显着增强。更具体地说,与对照组相比,50μg/mL的iturinA治疗组各项指标均降至24.84mm,0.33×106cfu/mL,菌落直径为5.86ng/mL,孢子的数量,以及T-2毒素的浓度,分别。此外,事实证明,在25μg/mL或50μg/mL时,iturinA会破坏细胞膜的完整性,并导致ROS显着增加。转录组学分析显示,随着伊特菌素A的治疗,氧化还原过程的基因上调,而菌丝生长的基因表达,细胞完整性,跨膜运输,能量代谢,其他人则被下调。更重要的是,Tri5基因簇受到显著抑制。本研究为探讨伊曲菌素A抑制尖孢酵母生长和T-2毒素合成的作用机制提供了新的见解,为伊曲菌素A在干制水产品保鲜中的应用提供了理论指导。
Fusarium oxysporum (F. oxysporum) is a common contaminant of dried fish, and the T-2 synthesis by this organism in dried fish products poses a serious public health risk. In this
study, we investigated the effects of iturin A, a cyclic lipopeptide produced by Bacillus subtilis, on the growth and synthesis of the T-2 toxin of F. oxysporum, and transcriptomics was conducted. Results showed that the inhibitory effect of iturin A on F. oxysporum was significantly enhanced with an increase in iturin A concentrations. More specifically, compared with the control group, all indexes in the iturin A treatment group with 50 μg/mL were decreased to 24.84 mm, 0.33 × 106 cfu/mL, and 5.86 ng/mL for the colony diameter, number of spores, and concentration of T-2 toxin, respectively. Furthermore, iturin A was proven to destroy the integrity of cell membranes and cause a significant increase in ROS at 25 μg/mL or 50 μg/mL. Transcriptomic analysis revealed that with the treatment of iturin A, the genes of the oxidation-reduction process were up-regulated, while the gene expression of mycelial growth, cell integrity, transmembrane transport, energy metabolism, and others were down-regulated. More importantly, the Tri5 gene cluster was significantly inhibited. This
study provided new insights into the mechanism for the inhibitory effect of iturin A on the growth and T-2 toxin synthesis of F. oxysporum and theoretical guidance for the application of iturin A in the preservation of dried aquatic products.