表面展示是一种在细胞膜上表达靶蛋白的重组技术,可应用于从病毒到哺乳动物细胞的几乎所有类型的生物实体。该技术已用于各种生物技术和生物医学应用,例如药物筛选,生物催化剂,图书馆筛选,定量分析,和生物传感器。在这次审查中,讨论了表面显示技术在生物传感器应用中的应用。详细来说,噬菌体展示,革兰氏阴性和革兰氏阳性细菌的细菌表面显示,和真核酵母细胞表面展示系统。该综述描述了表面显示系统在生物传感器应用中的优势,并总结了表面显示在生物传感器中的应用。
Surface display is a recombinant technology that expresses target proteins on cell membranes and can be applied to almost all types of biological entities from viruses to mammalian cells. This technique has been used for various biotechnical and biomedical applications such as drug screening, biocatalysts, library screening, quantitative assays, and biosensors. In this
review, the use of surface display technology in biosensor applications is discussed. In detail, phage display, bacterial surface display of Gram-negative and Gram-positive bacteria, and eukaryotic yeast cell surface display systems are presented. The
review describes the advantages of surface display systems for biosensor applications and summarizes the applications of surface displays to biosensors.