遗传因素在甲基苯丙胺依赖性易感性中起重要作用。在这行,与C激酶1（PICK1）和脑源性神经营养因子（BDNF）基因相互作用的蛋白质与甲基苯丙胺依赖（物质使用障碍）有关。因此，在一项病例对照研究中，我们调查了伊朗人群中PICK1和BDNF基因多态性与甲基苯丙胺依赖之间的关联.
在2015年至2018年期间，共招募了235例病例和204例对照。通过ARMS-PCR测定PICK1-rs713729，-rs2076369和BDNF-rs6265基因型。进行统计分析，使用SPSS20.0，阶段2.1.1程序以及SNP分析仪2.0。
在本研究中，在乘法和显性模型中包括PICK1-rs713729（OR1.38（CI1.08-1.52；P值0.004），和PICK1-rs2076369(OR1.31(CI1.10-1.56；P值0.002)乘法，显性和共显性模型与甲基苯丙胺滥用风险相关.此外，单倍型分析显示单倍型AG（OR2.50（CI1.50-4.16；P值0.0002）在显性，隐性和共显性模型，和单倍型TT（OR0.67（CI0.50-0.91；P值0.009）在具有甲基苯丙胺滥用风险的显性和共显性模型中。这项研究中的多态性都没有高水平的连锁不平衡。
我们的发现表明，PICK1基因多态性可能会影响我们人群中甲基苯丙胺依赖的风险。
Genetic factors play an important role in susceptibility to methamphetamine dependency. In this line, protein that interact with C-kinase-1 (PICK1) and brain-derived neurotrophic factor (BDNF) genes are linked to methamphetamine dependence (substance use disorder). Thus, in a
case-control study, we investigated the association between polymorphisms of PICK1 and BDNF genes and methamphetamine dependence in an Iranian population.
Total of 235 cases and 204 controls were recruited in a period between 2015 to 2018. The PICK1-rs713729, -rs2076369 and BDNF-rs6265 genotypes were determined via ARMS-PCR assay. Statistical analysis was performed, using SPSS 20.0, PHASE 2.1.1 program as well as SNP Analyzer 2.0.
In the present study, two polymorphisms including PICK1-rs713729 (OR 1.38 (CI 1.08-1.52; P-value 0.004) in multiplicative and dominant models, and PICK1-rs2076369 (OR 1.31 (CI 1.10-1.56; P-value 0.002) in multiplicative, dominant and co-dominant models were associated with the risk of methamphetamine abuse. Moreover, haplotype analysis showed a significant association of haplotype AG (OR 2.50 (CI 1.50-4.16; P-value 0.0002) in dominant, recessive and co-dominant models, and haplotype TT (OR 0.67 (CI 0.50-0.91; P-value 0.009) in dominant and co-dominant models with the risk of methamphetamine abuse. None of the polymorphisms in this study had a high level of linkage disequilibrium.
Our findings indicate that the PICK1 gene polymorphism might affect the risk of methamphetamine dependency in our population.
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