■鼻窦疾病在囊性纤维化(PwCF)患者中很普遍,并且可能是肺移植后(pTx)患者的气道感染库。囊性纤维化窦的微生物组成及其与慢性鼻-鼻窦炎(CRS)的关联相对未被探索。我们旨在检查PwCF和pTxPwCF中的窦道和下气道微生物组及其与CRS的关联。
■前瞻性单中心研究。
■共有31个性别和年龄(±2岁)与PwCF和pTxPwCF相匹配。
■收集人口统计学和临床数据以及鼻窦拭子和痰。使用鼻窦结果测试22(SNOT-22)(患者报告的结果)和Lund-McKay(计算机断层扫描鼻窦)评分评估CRS。样品经历通用16S核糖体RNA基因的MiSeqIllumina测序。
■总共包括31个PwCF(15pTxPwCF)。总气道微生物组组成以假单胞菌为主(46%),嗜血杆菌(14%),葡萄球菌(11%),链球菌(10%),和梭杆菌(6%)。在痰和窦样本中,Tx后样本的α多样性显着降低(P=0.005)。痰液之间的β-多样性存在显着差异(P=0.004),但不是按移植状态分类的窦(P=0.75)样本。虽然在首次访问时存在较高的β多样性与较低的SNOT-22评分相关的趋势,这没有达到显著性(P=.05)。
■窦和气道微生物在PwCF和pTxPwCF中有所不同,但是普遍存在的生物仍然是一致的。需要阐明微生物组与临床状态的关系,以更好地了解何时进行相应干预,以优化PwCF的鼻窦疾病管理。
UNASSIGNED: Sinus disease is prevalent in persons with cystic fibrosis (PwCF) and may be a reservoir of airway infection in postlung transplant (pTx) patients. The microbial composition of cystic fibrosis sinuses and its associations with chronic rhinosinusitis (CRS) is relatively unexplored. We aimed to examine the
sinus and lower airway microbiome and their associations with CRS in PwCF and pTxPwCF.
UNASSIGNED: Prospective single-centre
study.
UNASSIGNED: A total of 31 sex and age (±2 years) matched PwCF and pTxPwCF.
UNASSIGNED: Demographic and clinical data along with
sinus swabs and sputum were collected. CRS was assessed using Sinonasal Outcome Test-22 (SNOT-22) (patient reported outcome) and Lund-McKay (computed tomography sinus) scores. Samples underwent MiSeq Illumina sequencing of the universal 16S ribosomal RNA gene.
UNASSIGNED: A total of 31 PwCF (15 pTxPwCF) were included. Aggregate airways microbiome composition was dominated by Pseudomonas (46%), Haemophilus (14%), Staphylococcus (11%), Streptococcus (10%), and Fusobacterium (6%). α-diversity was significantly lower in post-Tx samples across both sputum and sinus samples (P = .005). β-diversity was significantly different between sputum (P = .004), but not
sinus (P = .75) samples by transplant status. While there was a trend in higher β-diversity associated with lower SNOT-22 score at time of first visit, this did not reach significance (P = .05).
UNASSIGNED: Sinus and airway microbiomes differed in PwCF and pTxPwCF, but the prevalent organisms remained consistent. Elucidating the relationship of the microbiome with clinical status to better understand when to intervene accordingly is needed to optimize sinus disease management in PwCF.