目的:评估营养药物混合物对免疫监视的急性影响。
方法:随机,双盲,安慰剂对照,交叉试验在11名健康受试者中进行.在服用安慰剂或500mg的UP360(来自芦荟的植物药混合物)之前和之后的1、2和3小时采集血样,Poriacocos,和迷迭香(APR提取物)。免疫表型和流式细胞术定量的单核细胞数量,NK细胞,NKT细胞,CD8+细胞毒性T细胞,γδT细胞,和总T细胞,CD25和CD69活化标志物的表达。血浆进行细胞因子检测,趋化因子,生长因子,以及超氧化物歧化酶和过氧化氢酶的酶活性。
结果:与安慰剂相比,从1小时开始,APR提取物的消耗引发了趋化因子水平的快速增加,包括IP-10(P<0.05)和MCP-1(P<0.1),在2小时(P<0.01)和3小时(P<0.05)达到峰值,分别。干细胞动员生长因子G-CSF在2小时时增加(P<0.05)。增强的免疫监视涉及单核细胞在1小时的短暂作用,其次是NKT细胞,CD8+细胞毒性T细胞,和γδT细胞在2-3小时。1小时时,单核细胞CD25表达增加(P<0.01),免疫细胞警觉性增加。NKT细胞(P<0.01),T细胞(P<0.05)。NKT细胞在2小时显示CD69上调(P<0.01)。抗氧化酶超氧化物歧化酶(P<0.05)和过氧化氢酶(P<0.01)在2小时时酶活性增加。
结论:服用APR提取物会引发趋化因子水平的急性变化。此外,免疫警觉性通过在多种类型的先天免疫细胞上表达激活标记而增加,其次是增强免疫监视和抗氧化保护。这表明自然免疫监测的有益增强,可能是通过肠介导的细胞因子释放和迷走神经通讯的组合,结合细胞保护免受氧化应激。
To evaluate the acute impact of a nutraceutical blend on immune surveillance.
A randomized, double-blind, placebo-controlled, cross-over
trial was conducted in 11 healthy subjects. Blood samples were taken immediately before and at 1, 2, and 3 hours after consuming placebo or 500 mg of UP360, which is a blend of botanicals from Aloe vera, Poria cocos, and rosemary (APR extract). Immunophenotyping and flow cytometry quantified numbers of monocytes, NK cells, NKT cells, CD8+ cytotoxic T cells, γδT cells, and total T cells, and expression of CD25 and CD69 activation markers. Plasma was tested for cytokines, chemokines, growth factors, and enzymatic activity of superoxide dismutase and catalase.
Compared to the placebo, consumption of APR extract triggered rapid increases in chemokine levels starting at 1 hour, including IP-10 (P<0.05) and MCP-1 (P<0.1), which peaked at 2 hours (P<0.01) and 3 hours (P<0.05), respectively. The stem cell-mobilizing growth factor G-CSF increased at 2 hours (P<0.05). Increased immune surveillance involved a transient effect for monocytes at 1 hour, followed by NKT cells, CD8+ cytotoxic T cells, and γδT cells at 2-3 hours. Increased immune cell alertness was seen at 1 hour by increased CD25 expression on monocytes (P<0.01), NKT cells (P<0.01), and T cells (P<0.05). NKT cells showed upregulation of CD69 at 2 hours (P<0.01). Increased enzymatic activity was seen at 2 hours for the antioxidant enzymes superoxide dismutase (P<0.05) and catalase (P<0.01).
Consumption of APR extract triggered acute changes to chemokine levels. In addition, immune alertness was increased via the expression of activation markers on multiple types of innate immune cells, followed by increased immune surveillance and antioxidant protection. This suggests a beneficial enhancement of natural immune surveillance, likely via a combination of gut-mediated cytokine release and vagus nerve communication, in combination with cellular protection from oxidative stress.