■氧化镁纳米颗粒（MgONPs）混合物对变形链球菌的抗菌和抗生物膜特性（S.mutans)，除了检查MgONPs清漆对保持牙齿颜色和抑制亚甲蓝扩散到牙釉质的影响。
■在去离子水（DW）中制备MgONPs混合物，无水乙醇（E），和松香与乙醇（RE），名为清漆。通过琼脂良好扩散测试了MgONPs混合物的抗菌和抗生物膜能力，菌落形成单位（CFU），和生物膜抑制微量滴定方法一式三份，并与氟化钠清漆（NaF）和氯己定漱口水（ChX）进行比较。使用分光光度计记录基本牙齿颜色。人工脱矿开始96小时。然后，将实验材料应用于相应的组，和10天的pH周期进行。然后，在相同的周围环境中记录颜色。通过将样品染色24小时来评估亚甲基蓝扩散。扩散测试是通过连接到立体显微镜的数码相机计算的。
■琼脂孔扩散测试在所有MgONPs混合物中都表现出明显的抑制作用（p=0.000），和与MgONPs-RE相关的最大抑制区直径。在CFU测试中观察到相同的发现。此外,2.5%,5%,与中度抑制生物膜形成的NaF和ChX组（p=0.003）相比，10％MgONPs-RE清漆显示出强的生物膜抑制能力（p=0.039）。研究表明，与NaF清漆相比，5％MgONPs-RE清漆可保持基本的牙齿颜色，并具有最小的亚甲蓝扩散（p=0.00）。
■评估作为混合物的MgONPs揭示了对S.mutans的抗菌和抗生物膜能力，具有较高的MgONPs-RE清漆效果。此外，在pH循环挑战和亚甲蓝扩散到牙釉质后，检查MgONPs-RE清漆对牙齿颜色保持的局部作用，证实了MgONPs-RE清漆在5％时的高性能。
UNASSIGNED: Antibacterial and antibiofilm properties of magnesium oxide nanoparticles (MgONPs) mixture assessed against Streptococcus mutans (S. mutans), in addition to examining MgONPs varnish impact on the preservation of the tooth color and inhibition of methylene blue diffusion to the enamel.
UNASSIGNED: MgONPs mixture was prepared in deionized water (DW), absolute ethanol (E), and
rosin with ethanol (RE), named varnish. The antibacterial and antibiofilm capacities of MgONPs mixtures were tested by agar well diffusion, colony-forming unit (CFU), and biofilm inhibition microtiter methods in triplicate and compared to sodium fluoride varnish (NaF) and chlorhexidine mouthwash (ChX). A spectrophotometer was used to record basic tooth color. The artificial demineralization was initiated for 96 h. Then, experimental materials were applied to the corresponding group, and 10-day pH cycles proceeded. Then, the color was recorded in the same ambient environment. The methylene blue diffusion was evaluated by staining the samples for 24 h. After that, the diffusion test was calculated by a digital camera attached to the stereomicroscope.
UNASSIGNED: The agar well diffusion test expressed a significant inhibition zone with all MgONPs mixtures (p = 0.000), and maximum inhibition zone diameter associated with MgONPs-RE. The same finding was observed in the CFU test. Additionally, 2.5%, 5%, and 10% MgONPs-RE varnish showed strong biofilm inhibition capacity (p = 0.039) compared to NaF and ChX groups that inhibit biofilm formation moderately (p = 0.003). The
study shows that the 5% MgONPs-RE varnish maintains basic tooth color with minimal methylene blue diffusion compared to NaF varnish (p = 0.00).
UNASSIGNED: Evaluating MgONPs as a mixture revealed antibacterial and antibiofilm capacity against S. mutans with a higher effect of MgONPs-RE varnish. Also, examining the topical effect of MgONPs-RE varnish on the preservation of the tooth color after pH cycle challenges and methylene blue diffusion to enamel confirmed the high performance of MgONPs-RE varnish at 5%.
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