杀虫剂已广泛用于控制对农业和人类健康有重大影响的害虫。有效的杀虫剂设计和抗性管理需要更好地了解杀虫剂目标。Pymetrozine,据报道,afidopyropen和flonicamid靶向位于昆虫脊索器官上的蛋白质,导致昆虫协调的破坏和进食的抑制。在这项研究中,我们系统地检查了六个果蝇突变体(五个瞬时受体电位通道和一个机械受体)对三种商业杀虫剂的敏感性,以确定对昆虫对杀虫剂的反应至关重要的受体亚基。我们的结果表明,iav1,nan36a和wtrw1突变体对pymetrozine和afidopyropen的易感性显着降低,但不是Flonicamid.三个突变雌性产生的卵数量明显少于w1118菌株。同时,nan36a和wtrw1突变体的所有雄性突变体和雌性的寿命明显短于作为对照的w1118菌株。然而,我们观察到wtrw1突变体中没有重力轴缺陷,并且pymetrozine消除了wtrw1突变体的反重力轴。使用热遗传学工具的行为测定进一步证实了生物测定结果,并支持Nan作为位于果蝇脊索神经元中的TRPV亚家族成员的想法,作为吡虫嗪的目标,干扰果蝇并导致运动缺陷和重力轴缺陷。一起来看,这项研究阐明了吡格雷嗪和阿非多吡酚与TRPV通道的相互作用,Nan和Iav,和TRPA频道,Wtrw.我们的研究提供了另一个证据,证明吡虫嗪和阿非多吡罗芬可能靶向南安,iav和wtrw渠道,并提供对可持续虫害管理战略发展的见解。
Insecticides have been widely used for the control of insect pests that have a significant impact on agriculture and human health. A better understanding of insecticide targets is needed for effective insecticide design and resistance management. Pymetrozine, afidopyropen and flonicamid are reported to target on proteins that located on insect chordotonal organs, resulting in the disruption of insect coordination and the inhibition of feeding. In this
study, we systematically examined the susceptibility of six Drosophila melanogaster mutants (five transient receptor potential channels and one mechanoreceptor) to three commercially used insecticides, in order to identify the receptor subunits critical to the insect\'s response to insecticides. Our results showed that iav1, nan36aand wtrw1 mutants exhibited significantly reduced susceptibility to pymetrozine and afidopyropen, but not to flonicamid. The number of eggs produced by the three mutant females were significantly less than that of the w1118 strain. Meanwhile, the longevity of all male mutants and females of nan36a and wtrw1 mutants was significantly shorter than that of the w1118 strain as the control. However, we observed no gravitaxis defects in wtrw1 mutants and the anti-gravitaxis of wtrw1 mutants was abolished by pymetrozine. Behavioral assays using thermogenetic tools further confirmed the bioassay results and supported the idea that Nan as a TRPV subfamily member located in Drosophila chordotonal neurons, acting as a target of pymetrozine, which interferes with Drosophila and causes motor deficits with gravitaxis defects. Taken together, this
study elucidates the interactions of pymetrozine and afidopyropen with TRPV channels, Nan and Iav, and TRPA channel, Wtrw. Our research provides another evidence that pymetrozine and afidopyropen might target on nan, iav and wtrw channels and provides insights into the development of sustainable pest management strategies.