目的:用免疫组织化学方法研究P2X受体亚型在人未成熟精子头部的定位,鼠标,仓鼠,和大鼠附睾帽。
方法:基础研究。
方法:大学医院。
方法:从接受转移性前列腺癌睾丸切除术的患者中获得了三种人附睾。
方法:P2X(1),P2X(2),P2X(3),和P2X(4)受体在精子上的免疫定位。
结果:在本研究中,P2X(1,2和3)受体定位在人类未成熟精子的头部进行了免疫组织化学证明,鼠标,仓鼠,和大鼠附睾帽。在小鼠附睾的精子头部也观察到P2X(4)受体免疫染色,仓鼠,和人类,但不是老鼠。随后在附睾尾囊的精子上有受体染色的丧失,除了在人类中,P2X(4)受体的染色持续存在。与花生凝集素(PNA)结合研究的比较表明,P2X受体位于顶体膜上。检查了P2X(5-7)受体,但发现不存在。
结论:受体亚型的位置变化与精子通过附睾时的功能上必需的形态和成熟变化是一致的,并暗示嘌呤能信号在精子成熟和可能的生育能力中的作用。
OBJECTIVE: To
study using immunohistochemistry the localization of P2X receptor subtypes on the head of immature sperm in the human, mouse, hamster, and rat caput epididymidis.
METHODS: Basic research.
METHODS: University-based hospital.
METHODS: Three human epididymides were obtained from patients undergoing orchidectomy for metastatic prostate cancer.
METHODS: P2X(1), P2X(2), P2X(3), and P2X(4) receptor immunolocalization on sperm.
RESULTS: In the present
study, P2X(1,2, and 3) receptor localization was immunohistochemically demonstrated on the head of immature sperm in the human, mouse, hamster, and rat caput epididymidis. P2X(4) receptor immunostaining was also observed on the head of sperm in the caput epididymidis of mice, hamsters, and humans, but not rats. There was a subsequent loss of receptor staining on sperm in the cauda epididymidis, except in humans where staining of P2X(4) receptors persisted. Comparision with peanut agglutinin (PNA) binding studies suggested the P2X receptors were located on the acrosome membrane. P2X(5-7) receptors were examined but found to be absent.
CONCLUSIONS: The change in localization of receptor subtypes is coincidental with the functionally essential morphologic and maturational changes seen in sperm as they travel through the epididymis, and is suggestive of a role for purinergic signaling in sperm maturation and possibly fertility.