山楂果实有酸甜的味道,除了对高脂血症有有益的治疗作用,高血压,和冠心病,使其广泛用于食品和临床医学。然而,它们的降压作用和抗高血压的潜在机制仍需阐明.本研究旨在通过药效学研究山楂及其单体金丝桃苷对自发性高血压大鼠的降压作用,血清代谢组学,和体内机制研究。山楂提取物和金丝桃苷干预7周后,血压明显降低。主动脉血管染色结果显示,经山楂提取物和金丝桃苷干预后,损伤明显改善。根据血清代谢组学研究,山楂提取物和金丝桃苷组调节血压的主要代谢途径是胆汁酸初级生物合成途径。定量实验证实,模型组胆汁酸水平与正常组相比有显著差异,而山楂组和金丝桃苷组接近正常组。基于胆汁酸-高血压相关指标的预测和文献,选择了9个参与胆汁酸代谢和炎症通路的基因进行进一步研究.FXR,TGR5、ET-1、NOS3、Akt1、TNF-α、山楂提取物和金丝桃苷组的Ptgs2、ACE2和KdrmRNA表达水平与模型组相比差异有统计学意义。总之,山楂提取物和金丝桃苷通过胆汁酸和炎症相关靶点对自发性高血压大鼠有降压作用。因此,山楂提取物有可能成为功能性食品或高血压的替代疗法。
Hawthorn fruits have a sweet and sour taste, besides having beneficial therapeutic effects on hyperlipidemia, hypertension, and coronary heart disease, making them widely used in food and clinical medicine. However, their hypotensive effects and potential mechanisms of anti-hypertension still need to be elucidated. This
study aims to explore the antihypertensive effect of hawthorn and its monomer hyperoside on spontaneously hypertensive rats through pharmacodynamics, serum metabolomics, and in vivo mechanism studies. After 7 weeks of intervention with hawthorn extract and hyperoside, the blood pressure was significantly reduced. Aortic vascular staining results showed that the injury was significantly improved after intervention with hawthorn extract and hyperoside. According to the serum metabolomics
study, the main metabolic pathway regulating blood pressure in hawthorn extract and hyperoside groups was the primary bile acid biosynthesis pathway. Quantitative experiments confirmed that the level of bile acid in the model group was significantly different from that in the normal group, while that in the hawthorn group and the hyperoside group was close to that in the normal group. Based on the prediction of bile acid-hypertension related targets and the literature, nine genes involved in bile acid metabolism and inflammatory pathways were selected for further
study. The FXR, TGR5, ET-1, NOS3, Akt1, TNF-α, Ptgs2, ACE2 and Kdr mRNA expression levels in the hawthorn extract and hyperoside groups were significantly different from those in the model groups. In summary, hawthorn extract and hyperoside have a hypotensive effect on spontaneously hypertensive rats through bile acid and inflammation related targets. Hence, hawthorn extract has the potential to become a functional food or an alternative therapy for hypertension.