目的探讨长链非编码RNA(lncRNA)SNHG7在膀胱癌组织和细胞系中的表达,并探讨其对膀胱癌细胞增殖的影响,凋亡和入侵过程。
收集膀胱癌组织和近癌组织。通过实时荧光定量PCR(RT-PCR)检测lncRNA-SNHG7在组织和细胞系中的表达。如通过用于检测干扰效率的RT-PCR检测的RNA干扰(siRNA)下调lncRNA-SNHG7的表达。CCK-8,流式细胞术和Transwell分析用于评估lncRNASNHG7对增殖的影响,膀胱癌细胞的凋亡和侵袭能力。通过westernblot测试lncRNA-SNHG7对上皮-间质转化(EMT)相关标志物的下调作用。
lncRNA-SNHG7在膀胱癌细胞系中上调。lncRNA-SNHG7的表达下调后,膀胱癌细胞的增殖减少,细胞凋亡增加,细胞的侵袭能力下降。E-cadherin的表达增加,但是N-钙粘蛋白的表达,波形蛋白和蜗牛减少。lncRNASNHG7在癌组织中的表达增加与肿瘤大小显著相关,转移和分期。
这项研究表明,lncRNA-SNHG7在膀胱癌组织和细胞中过表达。下调lncRNA-SNHG7可抑制膀胱癌细胞增殖,促进细胞凋亡,以及抑制细胞入侵,这为未来肿瘤靶向治疗提供了潜在的分子靶点。
The purpose of this study was to investigate the expression of long-chain non-coding RNA (lncRNA) SNHG7 in bladder cancer tissues and cell lines, and to explore its impact on bladder cancer cell proliferation, apoptosis and invasion processes.
Bladder cancer tissues and near-cancer tissues were collected. The expression of lncRNA-SNHG7 in tissues and cell lines was detected by real-time PCR (RT-PCR). The expression of lncRNA-SNHG7 was downregulated by RNA interference (siRNA) as detected by RT-PCR that was used to detect the interference efficiency. CCK-8, flow cytometry and Transwell assays were used to evaluate the effect of lncRNASNHG7 on the proliferation, apoptosis and invasion capability of bladder cancer cells. The downregulation effect of lncRNA-SNHG7 on Epithelial-Mesenchymal Transition (EMT) related markers was tested by westernblot.
lncRNA-SNHG7 was upregulated in bladder cancer cell lines. After the expression of lncRNA-SNHG7 was downregulated, the proliferation of bladder cancer cells was decreased, the apoptosis was increased, and the invasion ability of cells was decreased. The expression of E-cadherin was increased, but the expression of N-cadherin, vimentin and snail were decreased. Increased expression of lncRNASNHG7 in cancer tissues was significantly related to tumor size, metastasis and stage.
This study showed that lncRNA -SNHG7 is overexpressed in bladder cancer tissues and cells. Downregulation of lncRNA-SNHG7 can inhibit the proliferation of bladder cancer cells and promote apoptosis, as well as inhibit cell invasion, which provides a potential molecular target for future tumor targeted therapy.