背景:主要农作物的产量通常受到库容量和来源强度的限制。黄瓜是典型的棉子糖族低聚糖(RFOs)运输作物。非编码RNA和选择性聚腺苷酸化(APA)在植物生长过程的调控中起着重要作用。然而,它们在汇源调控中的作用尚未在RFOs易位物种中得到证实。
结果:这里,采用全转录组测序方法对不同汇强度下黄瓜叶片进行比较,也就是说,在底部的第12个节点没有携带果实的叶子(NFNLs)和携带果实的叶子(FNLs)。结果显示1101个差异表达(DE)mRNA,鉴定了79个DE长非编码RNA(lncRNAs)和23个DEmiRNAs,它们富含光合作用,能源生产和转换,植物激素信号转导,淀粉和碳水化合物代谢和蛋白质合成途径。潜在的共表达网络,DElncRNAs-DEmRNAs/DEmiRNAs-DEmRNAs,和竞争内源性RNA(ceRNA)调控模型(DElncRNAs-DEmiRNAs-DEmRNAs)与汇来源分配相关,是建造的。此外,37和48个DE基因,富含MAPK信号和植物激素信号转导通路,存在差异APA,和SPS(CsaV3_2G033300),GBSS1(CsaV3_5G001560),ERS1(CsaV3_7G029600),PNO1(CsaV3_3G003950)和Myb(CsaV3_3G022290)可能受FNL和NFNL之间的ncRNAs和APA调节,推测ncRNAs和APA参与黄瓜汇源碳分配基因表达的调控。
结论:这些结果揭示了mRNA之间的综合网络,ncRNAs,和APA在黄瓜汇源关系中的作用。我们的发现也为进一步研究ncRNA和APA提高黄瓜产量的分子机制提供了有价值的信息。
BACKGROUND: The yield of major crops is generally limited by sink capacity and source strength. Cucumber is a typical raffinose family oligosaccharides (RFOs)-transporting crop. Non-coding RNAs and alternative polyadenylation (APA) play important roles in the regulation of growth process in plants. However, their roles on the sink‒source regulation have not been demonstrated in RFOs-translocating species.
RESULTS: Here, whole-transcriptome sequencing was applied to compare the leaves of cucumber under different sink strength, that is, no fruit-carrying leaves (NFNLs) and fruit-carrying leaves (FNLs) at 12th node from the bottom. The results show that 1101 differentially expressed (DE) mRNAs, 79 DE long non-coding RNAs (lncRNAs) and 23 DE miRNAs were identified, which were enriched in photosynthesis, energy production and conversion, plant hormone signal transduction, starch and carbohydrate metabolism and protein synthesis pathways. Potential co-expression networks like, DE lncRNAs-DE mRNAs/ DE miRNAs-DE mRNAs, and competing endogenous RNA (ceRNA) regulation models (DE lncRNAs-DE miRNAs-DE mRNAs) associated with sink‒source allocation, were constructed. Furthermore, 37 and 48 DE genes, which enriched in MAPK signaling and plant hormone signal transduction pathway, exist differentially APA, and SPS (CsaV3_2G033300), GBSS1 (CsaV3_5G001560), ERS1 (CsaV3_7G029600), PNO1 (CsaV3_3G003950) and Myb (CsaV3_3G022290) may be regulated by both ncRNAs and APA between FNLs and NFNLs, speculating that ncRNAs and APA are involved in the regulation of gene expression of cucumber sink‒source carbon partitioning.
CONCLUSIONS: These results reveal a comprehensive network among mRNAs, ncRNAs, and APA in cucumber sink-source relationships. Our findings also provide valuable information for further research on the molecular mechanism of ncRNA and APA to enhance cucumber yield.