目的:尽管在治疗方面取得了进步,冠状动脉疾病(CAD)仍然是全球发病率和死亡率的主要原因。长链非编码RNA(lncRNAs)在动脉粥样硬化过程中发挥关键作用,ANRIL就是这样的lncRNA。本研究探讨了ANRIL多态性之间的关联(rs1333049:C>G,rs564398:T>C,和rs10757274:A>G)和土耳其患者组中的CAD以及CAD危险因素。
方法:该研究包括1285名参与者,包括736例诊断为CAD的患者(平均年龄=63.3±10.5岁)和549例非CAD对照(平均年龄=57.52±11.01岁)。使用qRT-PCR确定rs1333049、rs564398和rs10757274的基因型。
结果:rs1333049和rs10757274多态性的G等位基因携带与更高的Gensini评分相关,SYNTAX评分,总胆固醇,女性CAD患者和非CAD男性的甘油三酯水平。具有rs564398CC基因型的女性更易患CAD(p=0.02)和严重CAD(p=0.05)。此外,rs10757274和rs564398的G和T等位基因在高血压男性中更为普遍。此外,rs564398携带C等位基因与2型糖尿病(T2DM)风险降低相关(p=0.02).此外,在逻辑回归分析中,rs1333049C等位基因携带者降低T2DM(p=0.03)和CAD与T2DM复合(p=0.04)的风险。
结论:结论:所选择的ANRIL多态性与CAD的存在/严重程度和CAD危险因素相关,T2DM,和高血压。值得注意的是,这项研究,这是一项最大的样本量研究,研究了选定的多态性对土耳其个体中CAD及其危险因素的影响,支持以前对不同种族进行的研究的结果.
OBJECTIVE: Coronary artery disease (CAD) remains a leading cause of morbidity and mortality globally despite advancements in treatment. Long non-coding RNAs (lncRNAs) play crucial roles in the atherosclerotic process, with ANRIL being one such lncRNA. This
study explored the association between ANRIL polymorphisms (rs1333049:C > G, rs564398:T > C, and rs10757274:A > G) and CAD along with CAD risk factors in a Turkish patient group.
METHODS: The
study included 1285 participants, consisting of 736 patients diagnosed with CAD (mean age = 63.3 ± 10.5 years) and 549 non-CAD controls (mean age = 57.52 ± 11.01 years). Genotypes for rs1333049, rs564398, and rs10757274 were determined using qRT-PCR.
RESULTS: G allele carriage of both rs1333049 and rs10757274 polymorphisms were associated with higher Gensini score, SYNTAX score, total cholesterol, and triglyceride levels in female CAD patients and non-CAD males. Females with rs564398 CC genotype were more susceptible to CAD (p = 0.02) and severe CAD (p = 0.05). Moreover, the G and T alleles of rs10757274 and rs564398 were more prevalent among hypertensive males. Also, carrying the C allele for rs564398 was associated with a decreased risk for type 2 diabetes mellitus (T2DM) (p = 0.02). Besides, carriers of the rs1333049 C allele for decreased risk for T2DM (p = 0.03) and CAD complexed with T2DM (p = 0.04) in logistic regression analyses.
CONCLUSIONS: In conclusion, selected ANRIL polymorphisms were associated with CAD presence/severity and CAD risk factors, T2DM, and hypertension. Notably, this
study, the largest sample-sized
study examining the effects of selected polymorphisms on CAD and its risk factors among Turkish individuals, supported the findings of previous studies conducted on different ethnicities.