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Abstract：
BACKGROUND: Measurement of the circulating levels of long-non-coding RNAs (lncRNAs) in lupus nephritis (LN) patients could dramatically explore more insights about the disease pathogenesis. Hence, we aimed to quantify the level of expression of CTC-471J1.2 and NeST in LN patients and to correlate it with the disease activity.
METHODS: This case-control study was conducted on a group of children with juvenile LN attending to Mansoura University Children\'s Hospital (MUCH). Demographics, clinical, and laboratory findings were collected besides the measurement of lncRNAs by quantitative real-time PCR.
RESULTS: The expression level of lncRNAs-CTC-471J1.2 was significantly down-regulated in children with active LN versus inactive cases or controls. In contrast, the NeST was significantly up-regulated in active LN cases. A significant correlation was found between CTC-471J1.2 expression and LN activity parameters. Additionally, both lncRNAs showed a reasonable sensitivity and specificity in differentiation of active LN. A regression analysis model revealed that CTC-471J1.2 and NeST were independent predictors of active nephritis.
CONCLUSIONS: The expression level of circulatory lncRNAs-CTC-471J1.2 and NeST can be used as sensitive and specific biomarkers for active LN. Furthermore, both could serve as predictors for nephritis activity.
METHODS: This case-control study was conducted on a group of children with juvenile LN attending to Mansoura University Children\'s Hospital (MUCH). Demographics, clinical, and laboratory findings were collected besides the measurement of lncRNAs by quantitative real-time PCR.
RESULTS: The expression level of lncRNAs-CTC-471J1.2 was significantly down-regulated in children with active LN versus inactive cases or controls. In contrast, the NeST was significantly up-regulated in active LN cases. A significant correlation was found between CTC-471J1.2 expression and LN activity parameters. Additionally, both lncRNAs showed a reasonable sensitivity and specificity in differentiation of active LN. A regression analysis model revealed that CTC-471J1.2 and NeST were independent predictors of active nephritis.
CONCLUSIONS: The expression level of circulatory lncRNAs-CTC-471J1.2 and NeST can be used as sensitive and specific biomarkers for active LN. Furthermore, both could serve as predictors for nephritis activity.
摘要：
背景：在狼疮性肾炎（LN）患者中测量长链非编码RNA（lncRNA）的循环水平可以极大地探索有关疾病发病机理的更多见解。因此，我们旨在量化LN患者中CTC-471J1.2和NeST的表达水平,并将其与疾病活动相关联.
方法：本病例对照研究是在Mansoura大学儿童医院（MUCH）就诊的一组LN儿童中进行的。人口统计,临床,除了通过定量实时PCR测量lncRNAs之外，还收集了实验室发现。
结果：lncRNAs-CTC-471J1.2的表达水平在患有活动性LN的儿童与非活动性病例或对照中显著下调。相比之下,在活动性LN病例中NeST显著上调。发现CTC-471J1.2表达与LN活性参数之间存在显着相关性。此外,两种lncRNAs在活性LN的分化中显示出合理的敏感性和特异性。回归模型显示CTC-471J1.2和NeST是活动性肾炎的独立预测因子。
结论：循环lncRNAs-CTC-471J1.2和NeST的表达水平可用作活性LN的敏感和特异性生物标志物。此外，两者均可作为肾炎活动的预测因子。
方法：本病例对照研究是在Mansoura大学儿童医院（MUCH）就诊的一组LN儿童中进行的。人口统计,临床,除了通过定量实时PCR测量lncRNAs之外，还收集了实验室发现。
结果：lncRNAs-CTC-471J1.2的表达水平在患有活动性LN的儿童与非活动性病例或对照中显著下调。相比之下,在活动性LN病例中NeST显著上调。发现CTC-471J1.2表达与LN活性参数之间存在显着相关性。此外,两种lncRNAs在活性LN的分化中显示出合理的敏感性和特异性。回归模型显示CTC-471J1.2和NeST是活动性肾炎的独立预测因子。
结论：循环lncRNAs-CTC-471J1.2和NeST的表达水平可用作活性LN的敏感和特异性生物标志物。此外，两者均可作为肾炎活动的预测因子。
