这项初步研究的目的是确定病毒载量和在总长度上的分布,在短距离内,以及病毒感染动物肠道的不同层,用于未来的灭活研究。两只小牛,两只猪,两只山羊感染了牛病毒性腹泻病毒(BVDV),经典猪瘟病毒(CSFV),和小反刍动物病毒(PPRV),分别。在两个小牛的回肠中检测到均匀分布的最大BVDV病毒载量,平均滴度为6.0log10TCID50-eq/g。结肠和盲肠中的病毒载量分布不均匀。一头猪,均匀分布的CSFV平均在小肠和大肠中发现4.5和4.2log10TCID50-eq/g的病毒载量,分别。粘膜,粘膜下层,肌肉层/浆膜显示平均病毒载量为5.3、3.4和4.0log10TCID50-eq/g,分别。PPRV的均匀分布显示在两只山羊的回肠中,平均病毒载量为4.6log10TCID50-eq/g。平均粘膜,粘膜下层,肌肉层/浆膜病毒载量分别为3.5、2.8和1.7log10TCID50-eq/g,分别。这项初步研究为建立来自实验感染动物的肠道灭活实验提供了必要的数据,其中需要肠道病毒载量的水平和均匀分布。
The aim of this pilot
study was to determine viral loads and distribution over the total length, at short distances, and in the separate layers of the intestine of virus-infected animals for future inactivation studies. Two calves, two pigs, and two goats were infected with bovine viral diarrhoea virus (BVDV), classical swine fever virus (CSFV), and peste des petits ruminants virus (PPRV), respectively. Homogenously distributed maximum BVDV viral loads were detected in the ileum of both calves, with a mean titer of 6.0 log10 TCID50-eq/g. The viral loads in colon and caecum were not distributed homogenously. In one pig, evenly distributed CSFV mean viral loads of 4.5 and 4.2 log10 TCID50-eq/g were found in the small and large intestines, respectively. Mucosa, submucosa, and muscular layer/serosa showed mean viral loads of 5.3, 3.4, and 4.0 log10 TCID50-eq/g, respectively. Homogenous distribution of PPRV was shown in the ileum of both goats, with a mean viral load of 4.6 log10 TCID50-eq/g. Mean mucosa, submucosa, and muscular layer/serosa viral loads were 3.5, 2.8, and 1.7 log10 TCID50-eq/g, respectively. This pilot
study provides essential data for setting up inactivation experiments with intestines derived from experimentally infected animals, in which the level and the homogeneous distribution of intestinal viral loads are required.