未经证实:癌性微环境,以CD4+和CD8+T细胞浸润为特征,在调节癌症的进展和免疫治疗的治疗效率中起着至关重要的作用。然而,这些细胞及其相关细胞因子在胰腺癌(PC)肿瘤微环境中的分布尚不完全清楚。我们的研究旨在分析PC中CD4+IL-17+和CD8+T细胞的含量及其与患者临床病理特征和生存预后的关系。
UNASSIGNED:回顾性收集我院40例患者的PC组织和邻近组织。通过免疫组织化学测量组织学样品中CD4,IL-17和CD8的表达。使用Kaplan-Meier生存分析分析CD4、IL-17和CD8表达与临床特征的相关性。通过Cox比例风险模型检查影响PC结局的危险因素,然后建立了使用这些危险因素预测PC生存率的列线图.
UNASSIGNED:PC组织中CD4+IL-17+T细胞含量明显高于癌旁正常组织,CD8+T细胞数量显著低于癌旁正常组织(P<0.01)。PC组织中CD4+T细胞与TNM分期、淋巴结转移显著相关(P<0.05)。IL-17和CD8与组织学分级显著相关,TNM阶段,局部渗透,淋巴结转移(P<0.05)。CD4阳性和阴性患者的中位生存时间(MST)分别为13.2和21.4个月,分别。IL-17阳性和阴性患者的MSTs分别为10.4和24.8个月,分别。CD8阳性和阴性患者的MSTs分别为21.9和11.8个月,分别为(P<0.05)。Cox回归模型证明了TNM分期,淋巴结转移,CD4+IL-17+和CD8+T细胞影响PC预后(P<0.05)。列线图显示,TNMIII至IV期患者的生存概率降低,淋巴结转移,高CD4+IL-17+水平,和低CD8+表达。
未经证实:PC组织中的CD4+IL-17+和CD8+T细胞与TNM分期相关,淋巴结转移,和MST,并可作为新的PC预后指标。
UNASSIGNED: The cancerous microenvironment, characterized by the infiltration of CD4+ and CD8+ T cells, play a critical role in regulating the progression of cancer and treating efficiency of immunotherapy. However, the distribution of these cells and their associated cytokines in the tumor microenvironment of pancreatic cancer (PC) are not yet fully understood. Our
study aims to analyze the contents of CD4+IL-17+ and CD8+ T cells in PC and their relationship with the clinicopathological features and survival outcomes of patients.
UNASSIGNED: PC tissues and adjacent tissues were retrospectively collected from 40 patients in our hospital. The expression of CD4, IL-17, and CD8 in histological samples was measured by immunohistochemistry. The correlation between CD4, IL-17, and CD8 expression and clinical characteristics was analyzed using Kaplan-Meier survival analysis. The risk factors affecting the outcome of PC were examined by the Cox proportional hazards model, then a nomogram predicting the survival of PC using these risk factors was established.
UNASSIGNED: The content of CD4+IL-17+ T cells in PC tissues was significantly higher than that in adjacent normal tissues, while the number of CD8+ T cells was significantly lower than that in adjacent normal tissues (P<0.01). CD4+ T cells in PC tissues was significantly associated with TNM stage and lymph node metastasis (P<0.05). IL-17 and CD8 were significantly associated with histological grade, TNM stage, local infiltration, and lymph node metastasis (P<0.05). The median survival times (MSTs) of CD4 positive and negative patients were 13.2 and 21.4 months, respectively. The MSTs of IL-17 positive and negative patients were 10.4 and 24.8 months, respectively. The MSTs were 21.9 and 11.8 months for CD8 positive and negative patients, respectively (P<0.05). The Cox regression model demonstrated that TNM staging, lymph node metastasis, and CD4+IL-17+ and CD8+ T cells affected PC prognosis (P<0.05). The nomogram showed that the survival probability was reduced in patients with TNM stage III to IV, lymph node metastasis, high CD4+IL-17+ level, and low CD8+ expression.
UNASSIGNED: CD4+IL-17+ and CD8+ T cells in PC tissues are associated with TNM staging, lymph node metastasis, and MST, and can be used as new prognostic indicators for PC.