最近,基于线粒体基因细胞色素C氧化酶亚基1(COI)的DNA条形码已成为鉴定动物的广泛工具。它在人类寄生虫中的使用受到某些线虫组的限制,这些线虫很难扩增该基因。在这项研究中,我们展示了来自热带地区的稀有寄生虫的第一个COI条形码序列,Lagochilascaris小调,寄生了一个来自金塔纳罗奥的人类宿主,尤卡坦半岛南部,墨西哥。在感染部位观察到侧窦的乳突突破坏和小脑受累。在根治性乳突切除术和200mg口服阿苯达唑治疗63天后,病人完全康复了。根据针状体的长度和射精管的长度之间的比率确定了Lagochilascarisminor,鸡蛋的形状,和主机,以及与其同类的比较。感染方式未知,尽管可能是在直接接触鸡蛋或食用未煮熟的野肉之后。使用扫描电子显微镜证明成年人的形态,使用为微型甲壳类动物设计的半简并引物,从扩增中提供COI条形码的高质量序列。DNA条形码被证明是L.minor的可靠鉴定方法。将该物种的序列与从生命条形码数据库获得的81种a虫的序列进行比较,将其置于与Baylisscarisprocyonis最密切相关的独特进化枝中。未来使用DNA条形码对幼体和成年小乳杆菌进行诊断将允许识别其感染参数,传输,和精确的流行病学。在过去的十年中,尤卡坦半岛发生了lagochilaras虫病的报道,这表明它是该地区正在出现的人畜共患疾病。
Recently, DNA barcoding based on the mitochondrial gene cytochrome c oxidase subunit 1 (COI) has become a widespread tool to identify animals. Its use with parasites of humans has been limited with some groups of nematodes where the amplification of this gene has been difficult. In this study, we present the first COI barcode sequence of a rare parasite from tropical regions, Lagochilascaris minor, which parasitized a human host from Quintana Roo, southern Yucatán Peninsula, Mexico. Destruction of the mastoid apophysis in the lateral sinus and cerebellar involvement were observed at the site of infection. After a radical mastoidectomy and a treatment with 200 mg oral albendazole for 63 days, the patient completely recovered. Lagochilascaris minor was identified based on the ratio between length of spicules and ejaculatory duct, shape of eggs, and host, as well as comparison with its congeners. The mode of infection is unknown, although it could be after direct exposure to eggs or consumption of uncooked wild meat. Morphology of adults is demonstrated using scanning electron microscopy, and high-quality sequences of COI barcode are presented from amplifications using semi-degenerate primers designed for micro-crustaceans. DNA barcoding proved to be a reliable identification method for L. minor. A comparison of the sequences for this species with 81 ascaridoids obtained from the Barcode of Life Database places it in a unique clade most closely related to Baylisascaris procyonis. Future diagnosis of larval and adult stages of L. minor using DNA barcoding will allow the recognition of its infection parameters, transmission, and precise epidemiology.
Reports of lagochilascarosis in the Yucatán Peninsula have been occurred over the last decade, suggesting it is an emerging zoonotic disease in the region.