■人乳头瘤病毒(HPV)基因型和HPV感染的年龄分布对于国家疫苗接种和筛查计划计划至关重要。然而,在正常宫颈人群中提供这些数据的研究有限.本研究旨在探讨基于乙酸视觉检查(VIA)测试的临床正常宫颈女性的HPV基因型特征。
■2012年至2018年在雅加达的私人和公共医疗保健中心进行了为期7年的横断面研究。连续招募受试者。数据是通过回忆收集的,VIA,使用聚合酶链反应(PCR;SPF10-DEIA-LiPA25)方法进行HPVDNA检测。HPV基因分型程序包括DNA提取,使用HPVXpressMatrix试剂盒(PTKalGenDNA,东雅加达,印度尼西亚),和杂交。IBMSPSSver。20.0(IBM公司,Armonk,NY,美国)用于分析数据。
■共收集了1,397名受试者。在52名受试者中发现HPV-DNA检测阳性(3.7%);67%是单一的,33%是多重HPV感染。HPV52是最常见的HPV基因型,其次是HPV39、16、18、74、44、31、54和66。该人群中HPV感染最高的是31-40岁和41-50岁组。
■这项研究建议对31-50岁的女性进行有益的筛查。而不是“原始”非单价(HPV16、18、6、11、31、33、45、52、58),针对HPV16,18,6,11,31,39,44,52,74的HPV疫苗的不同“非共价”配方可能对印尼人群有用.然而,仍需要进一步的大样本量多中心研究.
UNASSIGNED: Human
papillomavirus (HPV) genotype and age distribution of HPV infection were crucial for the national vaccination and screening program planning. However, there was a limited
study providing these data in the normal cervix population. This
study aimed to explore the HPV genotypes profile of women with clinically normal cervix based on Visual Inspection of Acetic Acid (VIA) test.
UNASSIGNED: A 7-year cross-sectional
study was conducted from 2012 to 2018 in private and public health care centers in Jakarta. Subjects were recruited consecutively. Data were collected by anamnesis, VIA, and HPV DNA test using the polymerase chain reaction (PCR; SPF10-DEIA-LiPA25) method. HPV genotyping procedures include DNA extraction, PCR (SPF10-DEIA-LiPA25) using the HPV XpressMatrix kit (PT KalGen DNA, East Jakarta, Indonesia), and hybridization. The IBM SPSS ver. 20.0 (IBM Corp., Armonk, NY, USA) were used to analyze the data.
UNASSIGNED: A total of 1,397 subjects were collected. Positive HPV-DNA tests were found in 52 subjects (3.7%); 67% were single and 33% were multiple HPV infections. HPV 52 was the most frequently detected HPV genotype, followed by HPV 39, 16, 18 74, 44, 31, 54, and 66, respectively. The highest HPV infections in this population were in the 31-40 and 41-50 years old group.
UNASSIGNED: This
study suggested beneficial screening for women aged 31-50 years old. Instead of \"original\" nonavalent (HPV 16, 18, 6, 11, 31, 33, 45, 52, 58), the different \"nonavalent\" formula for HPV vaccines protecting against HPV 16, 18, 6, 11, 31, 39, 44, 52, 74 might be useful for Indonesian population. However, further multicenter studies with a huge sample size are still needed.