目的:许多临床前和临床研究最近证明了噬菌体疗法在治疗多药耐药细菌感染中的重要作用。然而,只有少数研究人员专注于监测噬菌体治疗过程中噬菌体介导的不良反应.本研究旨在证明CharlesFoster大鼠中噬菌体(肺炎克雷伯菌XDR菌株)的口服急性和亚急性毒性,特别是免疫反应和不良反应。
结果:将噬菌体以1010PFU/ml和1015PFU/ml的剂量作为单剂量(在急性毒性研究中)和每日剂量口服给予CharlesFoster大鼠28天(在亚急性毒性研究中)。通过口服管饲管给予1毫升噬菌体悬浮液。在任何实验动物和对照动物中均未观察到不良反应。此外,在整个研究期间,与对照组大鼠相比,观察到食物和水的摄入量以及体重均无明显变化。在噬菌体给药的第28天,收集血液以评估血液学,生化和细胞因子参数。数据表明在血液学上没有差异,与对照组相比,生化和细胞因子谱。在肉眼和组织病理学检查中,在任何治疗组中都没有观察到显著变化。细胞因子估计,白细胞介素-1β(IL-1β),IL-4,IL-6和IFN-γ,在实验过程中发现在正常范围内。
结论:结果得出结论,没有不良反应,包括对口服高剂量(1010PFU/ml)和极高剂量(1015PFU/ml)噬菌体混合物的严重有害影响。
结论:噬菌体的长期和长期口服给药也没有引起明显的免疫反应。
OBJECTIVE: Numerous pre-clinical and clinical studies have recently demonstrated the significant role of phage therapy in treating multidrug-resistant bacterial infections. However, only a few researchers have focused on monitoring the phage-mediated adverse reactions during phage therapy. The present
study aimed to demonstrated the oral acute and sub-acute toxicity of bacteriophages (Klebsiella pneumoniae XDR strain) in Charles Foster rats with special reference to immunological response and adverse effects.
RESULTS: Bacteriophages were orally administered in dosages of 1010 PFU/ml and a 1015 PFU/ml to Charles Foster rats as a single dose (in acute toxicity
study) and daily dosage for 28 days (in sub-acute toxicity
study). One millilitre suspension of bacteriophages was administered through the oral gavage feeding tube. No adverse effect was observed in any of the experimental as well as in the control animals. Furthermore, an insignificant change in food and water intake and body weight was observed throughout the
study period compared with the control group rats. On the 28th day of phage administration, blood was collected to estimate haematological, biochemical and cytokines parameters. The data suggested no difference in the haematological, biochemical and cytokine profiles compared to the control group. No significant change in any of the treatment groups could be observed on the gross and histopathological examinations. The cytokines estimated, interleukin-1 beta (IL-1β), IL-4, IL-6 and IFN-gamma, were found within the normal range during the experiment.
CONCLUSIONS: The results concluded that no adverse effect, including the severe detrimental impact on oral administration of high (1010 PFU/ml) and very high dose (1015 PFU/ml) of the bacteriophages cocktail.
CONCLUSIONS: The high and long-term oral administration of bacteriophages did not induce noticeable immunological response as well.