使用非固氮蓝细菌的可能性(Chroococussp。)通过培养皿实验测试了减少土壤硝酸盐污染的效果。应用0.03、0.05和0.08mg/cm2的嗜血球菌。通过吸收硝酸盐养分和促进土壤反硝化作用,有效去除土壤中的NO3--N。在0.05mg/cm2的最佳施用剂量下,44.06%,在初始NO3--N浓度为60、90和120mg/kg时,除去了36.89%和36.17%的NO3--N,分别。嗜血球菌释放的多糖。充当细菌反硝化的碳源,促进土壤盐分的降低,显着(p<0.05)刺激了反硝化细菌(反硝化细菌和反硝化细菌。)以及显着(p<0.05)提高了硝酸还原酶和亚硝酸还原酶的活性1.07-1.23和1.15-1.22倍,分别。嗜色球菌的应用。促进了土壤微生物群落中诺卡氏菌的优势,导致磷酸酶活性升高和有效磷含量增加。嗜色球菌的应用。正向调节属于Chitinophaga属的土壤细菌的生长,普雷沃氏菌和芽孢杆菌,这可能有助于增加土壤肥力通过产生有益的酶,如转化酶,脲酶和过氧化氢酶。迄今为止,这是首次验证非固氮蓝藻对硝酸盐污染土壤的修复效果。
The possibility of using the non-nitrogen-fixing cyanobacterium (Chroococcus sp.) for the reduction of soil nitrate contamination was tested through Petri dish experiments. The application of 0.03, 0.05 and 0.08 mg/cm2 Chroococcus sp. efficiently removed NO3--N from the soil through assimilation of nitrate nutrient and promotion of soil denitrification. At the optimal application dose of 0.05 mg/cm2, 44.06%, 36.89% and 36.17% of NO3--N were removed at initial NO3--N concentrations of 60, 90 and 120 mg/kg, respectively. The polysaccharides released by Chroococcus sp. acted as carbon sources for bacterial denitrification and facilitated the reduction of soil salinity, which significantly (p < 0.05) stimulated the growth of denitrifying bacteria (Hyphomicrobium denitrificans and Hyphomicrobium sp.) as well as significantly (p < 0.05) elevated the activities of nitrate reductase and nitrite reductase by 1.07-1.23 and 1.15-1.22 times, respectively. The application of Chroococcus sp. promoted the dominance of Nocardioides maradonensis in soil microbial community, which resulted in elevated phosphatase activity and increased available phosphorus content. The application of Chroococcus sp. positively regulated the growth of soil bacteria belonging to the genera Chitinophaga, Prevotella and Tumebacillus, which may contribute to increased soil fertility through the production of beneficial enzymes such as invertase, urease and catalase. To date, this is the first
study verifying the remediation effect of non-nitrogen-fixing cyanobacteria on nitrate-contaminated soil.