Interfaces between nacreous tablets are crucial to the outstanding mechanical properties of nacre in natural shells. Excellent research has been conducted to probe the effect of interfaces on strength and toughness of nacre, providing critical guidelines for the design of human-made laminated composites. This article reviews recent studies on interfacial mechanical behavior of nacre in red abalone and other shells, including experimental methods, analytical and numerical modeling. The discussions focus on the mechanical properties of dry and hydrated nacreous microstructures. The review concludes with discussions on representative studies of nacre-like composites with interfaces tuned using multiple approaches, and provides an outlook on improving the performance of composites with better interfacial controls.

Pearl powder is a well-known traditional Chinese medicine for a variety of indications from beauty care to healthcare. While used for over a thousand years, there has yet to be an in-depth understanding and review in this area. The use of pearl powder is particularly growing in the biomedical area with various benefits reported due to the active ingredients within the pearl matrix itself. In this review, we focus on the emerging biomedical applications of pearl powder, touching on applications of pearl powder in wound healing, bone repairing, treatment of skin conditions, and other health indications.

Nacre has achieved an excellent combination of strength and toughness through its unique brick-and-mortar structure of layered aragonite platelets bonded with biopolymers. Mimicking nacre has been considered as a practical way for the development of high-performance structural composites. Over the past years, many techniques have been developed to fabricate multifunctional nacre-mimetic materials, including freeze casting, layer-by-layer assembly, vacuum filtration, 3D printing and so on. Among them, freeze casting, especially bidirectional freeze casting, as an environmentally friendly and scalable method, has attracted extensive attention recently. In this review, we begin with the introduction and discussion of various fabrication techniques comparing their advantages and disadvantages, focusing on the most recent advances of the bidirectional freeze casting technique. Then, we summarize representative examples of applying the bidirectional freeze casting technique to assemble various building blocks into multifunctional nacre-mimetic materials and their wide applications. At the end, we discuss the future direction of using bidirectional freeze casting to make nacre-mimetic materials.

Through billions of years of evolution and natural selection, biological systems have developed strategies to achieve advantageous unification between structure and bulk properties. The discovery of these fascinating properties and phenomena has triggered increasing interest in identifying characteristics of biological materials, through modern characterization and modeling techniques. In an effort to produce better engineered materials, scientists and engineers have developed new methods and approaches to construct artificial advanced materials that resemble natural architecture and function. A brief review of typical naturally occurring materials is presented here, with a focus on chemical composition, nano-structure, and architecture. The critical mechanisms underlying their properties are summarized, with a particular emphasis on the role of material architecture. A review of recent progress on the nano/micro-manufacturing of bio-inspired hybrid materials is then presented in detail. In this case, the focus is on nacre and bone-inspired structural materials, petals and gecko foot-inspired adhesive films, lotus and mosquito eye inspired superhydrophobic materials, brittlestar and Morpho butterfly-inspired photonic structured coatings. Finally, some applications, current challenges and future directions with regard to manufacturing bio-inspired hybrid materials are provided.

The wide diversity of biogenic minerals that is found in nature, each with its own morphology, mechanical properties and composition, is remarkable. In order to produce minerals that are optimally adapted for their function, biomineralisation usually occurs under strict cellular control. This control is exerted by specialised proteins and polysaccharides that assemble into a 3-dimensional organic matrix framework, forming a microenvironment where mineral deposition takes place. Molluscs are unique in that they use a striking variety of structural motifs to build their shells, each made of crystals with different morphologies and different calcium carbonate polymorphs. Much of want is known about mollusc shell formation comes from studies on the nacreous layer, or mother-of-pearl. In this review, we discuss two existing models on the nucleation of aragonite crystals during nacre formation: heteroepitaxial nucleation and mineral bridges. The heteroepitaxial nucleation model is based on the identification of chemical functional groups and aragonite-nucleating proteins at the centre of crystal imprints. It proposes that during nacre formation, each aragonite tablet nucleates independently on a nucleation site that is formed by acidic proteins and/or glycoproteins adsorbed on the chitin scaffold. The mineral bridges model is based on the identification of physical connections between the crystals in a stack, which results in a large number of crystals across several layers sharing the same crystallographic orientation. These observations suggest that there is one nucleation event per stack of tablets. Once the first crystal nucleates and reaches the top interlamellar matrix, it continues growing through pores, giving rise to the next layer of nacre, subsequently propagating into a stack. We compare both models and propose that they work in concert to control crystal nucleation in nacre. De novo crystal nucleation has to occur at least once per stack of aligned crystals, and is induced by nucleation sites. We suggest that further growth is controlled both by mineral bridges and nucleation sites. Finally, we discuss the role of amorphous calcium carbonate precursor in nacre formation.

Natural selection and evolution develop a huge amount of biological materials in different environments (e.g. lotus in water and opuntia in desert). These biological materials possess many inspiring properties, which hint scientists and engineers to find some useful clues to create new materials or update the existing ones. In this review, we highlight some well-studied (e.g. nacre shell) and newly-studied (e.g. turtle shell) natural materials, and summarize their hierarchical structures and mechanisms behind their mechanical properties, from animals to plants. These fascinating mechanisms suggest to researchers to investigate natural materials deeply and broadly, and to design or fabricate new bio-inspired materials to serve our life.

The performance of man-made materials can be improved by exploring new structures inspired by the architecture of biological materials. Natural materials, such as nacre (mother-of-pearl), can have outstanding mechanical properties due to their complicated architecture and hierarchical structure at the nano-, micro- and meso-levels which have evolved over millions of years. This review describes the numerous experimental methods explored to date to produce composites with structures and mechanical properties similar to those of natural nacre. The materials produced have sizes ranging from nanometres to centimetres, processing times varying from a few minutes to several months and a different range of mechanical properties that render them suitable for various applications. For the first time, these techniques have been divided into those producing bulk materials, coatings and free-standing films. This is due to the fact that the material\'s application strongly depends on its dimensions and different results have been reported by applying the same technique to produce materials with different sizes. The limitations and capabilities of these methodologies have been also described.