未经批准:前列腺癌根治术,局部前列腺癌(PC)的标准管理方法,可能会引起与免疫调节作用相关的应激反应。假设区域麻醉通过最小化神经内分泌手术应激反应来降低手术的免疫效应。从而减轻肿瘤细胞的播散。我们的主要目的是研究在动物模型上使用脊髓块是否会减弱PC肿瘤细胞的播散。我们还评估了循环NK细胞的数量以及炎症和抗炎细胞因子的量。
未经证实:皮下肿瘤模型,使用用荧光素酶产生基因(PC-3M-luc-C6)转染的PC-3M细胞系。在适当的肿瘤建立后和肿瘤转移之前,动物在全身麻醉或联合麻醉(全身麻醉和脊髓麻醉)下接受肿瘤切除手术。对照组仅全身麻醉。
UNASSIGNED:具有PC-3M-luc-C6细胞的皮下肿瘤模型在35天后可有效引起远处转移。与接受联合麻醉的组相比,仅在全身麻醉下接受手术的动物中循环肿瘤细胞的数量增加。白细胞介素6水平在各组均有差异,随着全身麻醉组的增加。
UNASSIGNED:我们的研究结果表明,椎管内麻醉和全身麻醉联合使用可能会减弱对先天肿瘤免疫的抑制,这可能与手术后神经内分泌反应的减少有关。
未经批准:动物伦理委员会1332/2019。
UNASSIGNED: Radical prostatectomy, a standard management approach for localized Prostate Cancer (PC), may cause a stress response associated with immune modulating effects. Regional anesthesia was hypothesized to reduce the immune effects of surgery by minimizing the neuroendocrine surgical stress response, thus mitigating tumor cells dissemination. Our primary objective was to investigate whether the use of spinal blocks attenuates PC tumor cells dissemination on an animal model. We also assessed the number of circulating NK cells and the amount of inflammatory and anti-inflammatory cytokines.
UNASSIGNED: A subcutaneous tumor model, with PC-3M cell line transfected with a luciferase-producing gene (PC-3M-luc-C6) was used. After proper tumor establishment and before tumors became metastatic, animals were submitted to tumor excision surgeries under general or combined (general and spinal) anesthesia. A control group was only anesthetized with general anesthesia.
UNASSIGNED: The subcutaneous tumor model with PC-3M-luc-C6 cells was effective in causing distant metastasis after 35 days. The number of circulating tumor cells increased in animals that underwent surgery under general anesthesia alone compared to the group submitted to combined anesthesia. Interleukin 6 levels were different in all groups, with increase in the general anesthesia group.
UNASSIGNED: Our results suggest that combination of spinal and general anesthesia may attenuate the suppression of innate tumor immunity and it might be related to a reduction in the neuroendocrine response to surgery.
UNASSIGNED: Animal Ethics Committee 1332/2019.