在过去的十年里,丹麦博物馆中广泛的真菌生长与气候变化平行,挑战职业健康和遗产保护。当博物馆努力将相对湿度控制在60%以下时,这种增长是出乎意料的。它应该超过75%,风险增长。丹麦的一项案例研究发现,嗜干曲霉能够在博物馆存储库中的低相对湿度下生长。这项横断面研究旨在检查来自Aspergillus部分Restricti的干性生长是否已成为丹麦博物馆资料库中全国性分布的新型污染物,这些污染物旨在根据国际环境对遗产收藏的建议来控制相对湿度。该研究调查了丹麦国家博物馆和分布在丹麦各地的八个大型国家认可博物馆。它基于600个拭子和胶带提升样品以及60个MAS100-Eco和过滤器空气样品,分析了真菌的培养和形态鉴定,大染料-桑格测序,CaM-纳米孔和ITS-Illumina扩增子测序。该研究显示了七种嗜干曲霉的生长:A.conicus,A.家畜,A.glabripes,A.嗜盐,A.卵黄囊,A.penicilloides,A.vitricola,其中三个是丹麦新来的,和13种耐干性曲霉。其他真菌物种没有生长。多重检测方法提供了广泛的表征;然而,根据分析方法的不同,检测到的物种存在差异。培养和大染料桑格测序显示了最高的曲霉多样性,检测到17个物种;CaM-纳米孔扩增子测序检测到12个物种;ITS-illumina扩增子测序检测到两个物种,但总体多样性最高。耕种,其次是大染料Sanger和CaM-扩增子测序,证明了最高的合规性。该研究得出的结论是,嗜干性曲霉的生长在全国范围内分布,并表明来自曲霉科Restricti的物种是气候控制的博物馆资料库中的新型污染物。在可持续解决方案中保护职业健康和遗产保护研究,避免博物馆藏品中的嗜酸性生长是最重要的。
In the last decade, extensive fungal growth has developed in Danish
museums parallel to climate change, challenging occupational health and heritage preservation. The growth was unexpected as the
museums strived to control relative humidity below 60 %, and it should exceed 75 % to risk growth. A Danish case
study found xerophilic Aspergillus species able to grow at low relative humidity in a museum repository. This cross-sectional
study aimed to examine whether xerophilic growth from Aspergillus section Restricti has become a novel contaminant nationally distributed in Danish museum repositories striving to control relative humidity according to international environmental recommendations for heritage collections. The
study examined The National Museum of Denmark and eight large State Recognized
museums distributed throughout Denmark. It was based on 600 swab and tape-lift samples and 60 MAS100-Eco and filter air samples analyzed for fungi with cultivation and morphological identification, Big-Dye-Sanger sequencing, CaM-Nanopore and ITS-Illumina amplicon sequencing. The study showed growth from seven xerophilic Aspergillus species: A. conicus, A. domesticus, A. glabripes, A. halophilicus, A. magnivesiculatus, A. penicilloides, A. vitricola, of which three are new to Denmark, and 13 xerotolerant Aspergillus species. There was no growth from other fungal species. The multiple detection approach provided a broad characterization; however, there was variance in the detected species depending on the analysis approach. Cultivation and Big-Dye Sanger sequencing showed the highest Aspergillus diversity, detecting 17 species; CaM-Nanopore amplicon sequencing detected 12 species; and ITS-illumina amplicon sequencing detected two species but the highest overall diversity. Cultivation, followed by Big-Dye Sanger and CaM-amplicon sequencing, proved the highest compliance. The study concluded that xerophilic Aspergillus growth is nationally distributed and suggests species from Aspergillus section Restricti as a novel contaminant in climate-controlled museum repositories. To safeguard occupational health and heritage preservation research in sustainable solutions, avoiding xerophilic growth in museum collections is most important.