当诊断测试不符合负担能力的一个或多个必要标准时,传染病的诊断无效,可访问性,和准确性。世界卫生组织进一步澄清了这些标准的一组标准,其首字母缩写为ASSURED(负担得起,敏感,Specific,用户友好,快速和强大的,无设备且可交付给最终用户)。数字时代的进步导致了对保证标准的修订:实时连接,易于收集样本,经济实惠,敏感,Specific,用户友好,快速和强大的,无设备或简单,并可交付最终用户。已经开发了许多诊断测试,旨在满足REASSURED标准;然而,他们中的大多数只检测到一个目标。随着综合征性感染的进展,共感染和当前的抗菌素耐药性挑战,现在,对多路诊断的需求比以往任何时候都更加重要。这篇综述总结了当前用于多路检测和预测的诊断技术,这些方法有望检测多个目标并满足所有REASSURED标准。
The diagnosis of infectious diseases is ineffective when the diagnostic test does not meet one or more of the necessary standards of affordability, accessibility, and accuracy. The World Health Organization further clarifies these standards with a set of criteria that has the acronym ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free and Deliverable to end-users). The advancement of the digital age has led to a revision of the ASSURED criteria to REASSURED: Real-time connectivity, Ease of specimen collection, Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free or simple, and Deliverable to end-users. Many diagnostic tests have been developed that aim to satisfy the REASSURED criteria; however, most of them only detect a single target. With the progression of syndromic infections, coinfections and the current antimicrobial resistance challenges, the need for multiplexed diagnostics is now more important than ever. This
review summarizes current diagnostic technologies for multiplexed detection and forecasts which methods have promise for detecting multiple targets and meeting all REASSURED criteria.