多环芳烃(PAHs)对人类和海洋动物都有毒。本研究评估了PAHs对从Bizerte泻湖收集的中底线虫迁徙行为的影响,突尼斯。实验持续了15天,并在开放的微观世界中进行,其中包括一个较低的,被污染和鞋面,未被污染的隔间。使用了三种治疗方法,对于他们每个人都设置了未经处理的对照:被chrysene污染的沉积物(116ngg-1干重(DW),用菲(116ngg-1DW)和两者的混合物。结果表明,下层的发散性和丰度显着下降,与上部区域相比,受污染的隔间。结果还强调,在增加的压力下,一些物种的数量逐渐增加,这些被认为是耐PAH的物种,例如小齿齿,其他一些偶尔增加的数量被认为是机会物种,例如副相瘤和数量逐渐减少的物种,如美托霍莱姆斯和Steineriasp。,Terschellingia.Longicaudata,和Oncholaimellussp.被归类为PAH敏感。此外,观察到,在T.longicaudata的男性和女性孕妇暴露于29、58和87ngg-1的chrysene和菲的DW后,生化生物标志物的活性增加,而后者人口统计学类别的脆弱性更高。此外,对于这两种类型的PAHs,均观察到女性生育力显著下降,咽部吸吮力增加.性别比例也明显失衡,对男性有利,这表明chrysene和菲也会影响T.longicaudata的激素系统。这些PAHs的高亲和力及其与种系发育蛋白3(GLD-3)和性别决定蛋白(SDP)的分子相互作用可能证明了这些结果并解释了毒物动力学属性。
Polycyclic aromatic hydrocarbons (PAHs) are toxic for humans and marine fauna alike. The current study assessed the impact of PAHs on the migratory behaviour of meiobenthic nematodes collected from the Bizerte lagoon, Tunisia. The experiment lasted for 15 days and was carried in open microcosms, which comprised a lower, contaminated and an upper, uncontaminated compartment. Three treatments were used, for each of them an untreated control was set up: sediment contaminated with chrysene (116 ng g-1 dry weight (DW), with phenanthrene (116 ng g-1 DW) and a mixture of both. The results showed a significant decrease in diversty and abundance in the lower, contaminated compartments compared to the upper zones. The results also highlighted that under an increased stress some species progressively increased in number, these were considered PAH-tolerant species such as Odontophora villoti, some others had an occasionally increased in number were considered as opportunistic species, such as Paracomesoma dubium and the species that showed a progressive decreased in number, such as Metoncholaimus pristiurus and Steineria sp., Terschellingia. longicaudata, and Oncholaimellus sp. were classified as PAH-sensitive. Moreover, an increase in the activity of biochemical biomarkers was observed following the exposure of males and gravid females of T. longicaudata to 29, 58 and 87 ng g-1 DW of chrysene and phenanthrene paralleled by a higher vulnerability of the latter demographic category. Besides, a significant decrease in fertility of females and an increase in pharyngeal sucking power were observed for both types of PAHs considered. The sex ratio was also significantly imbalanced in the favor of males, which suggest that chrysene and phenanthrene affect also the hormone system of T. longicaudata. The high affinities of these PAHs and their molecular interactions with both germ line development protein 3 (GLD-3) and sex-determining protein (SDP) may justify these results and explain the toxicokinetic attributes.