人脂肪来源的基质/干细胞(hASC)广泛用于生理相关的人脂肪组织的体外建模。这些模型可用于开发用于软组织再生的组织构造和用于药物发现的三维(3D)微生理系统(MPS)。在这次系统审查中,我们报告了使用3DMPS进行脂肪组织工程的hASC培养和评估方法的现状。我们的搜索工作导致识别出184条独立记录,其中27个被确定为与本审查的目标最相关。我们的结果表明,对于hASC培养和评估人类脂肪组织生理相关体外模型的产生方法缺乏共识。很少有研究评估不同3D培养条件对hASC脂肪生成的影响。此外,用于体外表征脂肪组织功能的测定法的使用有限。这项研究的结果表明需要更标准化的培养方法和对体外组织功能的进一步分析。这些将是必要的,以验证3DMPS作为体外模型的实用性,精炼,并取代药物发现监管过程中的体内实验。
Human adipose-derived stromal/stem cells (hASC) are widely used for in vitro modeling of physiologically relevant human adipose tissue. These models are useful for the development of tissue constructs for soft tissue regeneration and 3-dimensional (3D) microphysiological systems (MPS) for drug discovery. In this systematic
review, we report on the current state of hASC culture and assessment methods for adipose tissue engineering using 3D MPS. Our search efforts resulted in the identification of 184 independent records, of which 27 were determined to be most relevant to the goals of the present
review. Our results demonstrate a lack of consensus on methods for hASC culture and assessment for the production of physiologically relevant in vitro models of human adipose tissue. Few studies have assessed the impact of different 3D culture conditions on hASC adipogenesis. Additionally, there has been a limited use of assays for characterizing the functionality of adipose tissue in vitro. Results from this study suggest the need for more standardized culture methods and further analysis on in vitro tissue functionality. These will be necessary to validate the utility of 3D MPS as an in vitro model to reduce, refine, and replace in vivo experiments in the drug discovery regulatory process.