UNASSIGNED: Digital denture fabrication became an alternative method to conventional denture fabrication. However reviewing the antimicrobial performance of newly introduced digital fabrication methods in comparison to the conventional method is neglected. Aim of study: this review was to compare the antiadherence properties of various CAD-CAM subtractive (milled), additive (3D printed) conventional denture base resins. In order to answer the developed PICO question: \"Does CAD-CAM milled and 3D printed denture base resins have microbiological antiadherence properties over the conventional ones?\" We included comparative studies on digitally fabricated Denture base resins with conventionally fabricated one in term of microbial adhesion.
UNASSIGNED: All in vitro studies investigated the microbial adherence to CAD-CAM milled and 3D printed denture base resins in comparison to conventional were searched in the PubMed, Web of Sciences, and Scopus databases up to December 2023.
UNASSIGNED: Fifteen studies have been investigated the microbial adhesion to milled and 3D printed denture base resins. CAD-CAM milled resins significantly decreased the microbial adhesion when compared with the conventional resins and 3D printed resins, while the later showed a high tendency for microbial adhesion. The addition of antifungal agents to 3D printed resins significantly reduced C. albicans adhesion. In terms of 3D printing parameters, printing orientation affected adherence while printing technology had no effect on microbial adhesion.
UNASSIGNED: Denture base materials and fabrication methods significantly affect the microbial adhesion. CAD-CAM milled denture base resins demonstrated low microbial adhesion. 3D-printed resins showed high tendency for C. albicans adhesion. The antiadherent properties of 3D-printed resins can be improved by incorporating antifungal agents or changing the printing parameters, but further investigations are required to validate these modifications.

This review aimed to identify the influence of antimicrobial and cleaning agents on surface characteristics such as surface free energy (SFE) and wettability, and microbial adhesion in polymethylmethacrylate (PMMA) for denture base. The review question, based on PICO, was: \"Does intervention with antimicrobial and cleaning agents in PMMA influence the surface free energy, wettability, and consequently the microbial adhesion?\" and the protocol was registered in Open Science Framework (osf. io/v3xgn). The search was performed in PubMed, Lilacs, Embase, Scopus, and Science Direct databases, using the terms: (\"acrylic resin\" OR PMMA) AND (antimicrobial OR antibacterial) AND (\"electrostatic interaction\" OR surface free energy) AND (biofilm OR \"bacteria adhesion\"), and resulted in 462 articles, of which 7 were included. The antimicrobials polypara-xylylene, carboxybetaine methacrylate, ethylene glycol methacrylate phosphate, and deposition of F and Ag ions in PMMA influenced the SFE and wettability. Denture cleaners reduced microbial adhesion. Five of the included studies evaluated the microbial adhesion, however, only two observed a direct relationship between SFE, wettability, and microbial adhesion. It was concluded that the intervention with antimicrobial and cleaning agents in PMMA can interfere in SFE and surface wettability, but no correlation was observed between microbial adhesion and these surface characteristics in PMMA.