近年来出现了世界性的传染病问题,和抗菌剂对于减少疾病的出现至关重要。然而,病原菌中多药耐药(MDR)菌株的发展和分布,如大肠杆菌,金黄色葡萄球菌,伤寒沙门氏菌和科塞利柠檬酸杆菌,已成为社会健康的主要危害。精油可以作为一种有前途的工具,作为一种天然药物来对抗这些细菌的问题。本研究旨在研究茶树(互叶白千层(少女和贝切)Cheel)的抗菌效果,迷迭香(迷迭香),桉树(桉树。),和薰衣草(LavandulaangustifoliaMill)精油。筛选了精油对4种病原菌的抗菌性能,大肠杆菌,金黄色葡萄球菌,S.Tyhpi,还有C.Koseri,和两个参考细菌菌株,而为了测试,采用琼脂孔扩散法。对精油进行气相色谱(GC)和气相色谱-质谱(GC-MSD)分析。得到的结果表明,互叶菊精油中松油烯-4-醇含量最丰富,1,8-桉树脑中的R.officinalis和E.斜精油,和L.angustifolia精油在α-terpinyl乙酸酯。此外,茶树精油中存在的主要生物活性化合物富含α-pine烯(18.38%),柠檬烯(7.55%)和γ-萜品烯(14.01%)。迷迭香精油中含有丰富的α-pine烯(8.38%)和柠檬烯(11.86%);桉树精油中含有显著浓度的α-pine烯(12.60%),对异丙基苯(3.24%),柠檬烯(3.87%),和γ-萜品烯(7.37%),而薰衣草的精油富含芳樟醇(10.71%),芳樟醇乙酸酯(9.60%),乙酸α-萜品酯(10.93%),和卡必醇(13.05%)生物活性化合物,分别。从体外研究获得的结果表明,大多数精油表现出抗菌性能。在测试的精油中,茶树被发现具有最强的抗菌活性。记录的伤寒沙门氏菌的MIC为6.2mg/mL,3.4毫克/毫升C.koseri,3.1mg/mL大肠杆菌,和2.7mg/mL的大肠杆菌ATCC25922,与互花分枝杆菌相比。同样,只有金黄色葡萄球菌ATCC25923对R.officinalis(1.4mg/mL)显示出抗微生物活性,E.斜(2.9mg/mL),和L.angustifolia(2.1mg/mL)。根据获得的结果,可以得出结论,茶树精油可能用作治疗由测试病原体引起的传染病的生态抗菌剂。
The worldwide problem of infectious diseases has appeared in recent years, and antimicrobial agents are crucial in reducing disease emergence. Nevertheless, the development and distribution of multidrug-resistant (MDR) strains in pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Salmonella Typhi and Citrobacter koseri, has become a major society health hazard. Essential oils could serve as a promising tool as a natural drug in fighting the problem with these bacteria. The current
study aimed to investigate the antimicrobial effectiveness of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel), rosemary (Rosmarinus officinalis L.), eucalyptus (Eucalyptus obliqua L\'Hér.), and lavender (Lavandula angustifolia Mill) essential oils. The antimicrobial properties of essential oils were screened against four pathogenic bacteria, E. coli, S. aureus, S. Tyhpi, and C. koseri, and two reference bacterial strains, while for the testing, the agar well diffusion method was used. Gas chromatography (GC) and gas chromatography-mass spectrometric (GC-MSD) analyses were performed on essential oils. The obtained results showed that M. alternifolia essential oil is the richest in terpinen-4-ol, R. officinalis and E. oblique essential oils in 1,8-cineole, and L. angustifolia essential oil in α-terpinyl acetate. In addition, the main bioactive compounds present in the essential oil of tea tree are rich in α-pinene (18.38%), limonene (7.55%) and γ-terpinene (14.01%). The essential oil of rosemary is rich in α-pinene (8.38%) and limonene (11.86%); eucalyptus essential oil has significant concentrations of α-pinene (12.60%), p-cymene (3.24%), limonene (3.87%), and γ-terpinene (7.37%), while the essential oil of lavender is rich in linalool (10.71%), linalool acetate (9.60%), α-terpinyl acetate (10.93%), and carbitol (13.05%) bioactive compounds, respectively. The obtained results from the in vitro
study revealed that most of the essential oils exhibited antimicrobial properties. Among the tested essential oils, tea tree was discovered to demonstrate the strongest antimicrobial activity. The recorded MIC of S. Typhi was 6.2 mg/mL, 3.4 mg/mL of C. koseri, 3.1 mg/mL of E. coli, and 2.7 mg/mL of E. coli ATCC 25922, compared to M. alternifolia. Similarly, only S. aureus ATCC 25923 showed antimicrobial activity towards R. officinalis (1.4 mg/mL), E. oblique (2.9 mg/mL), and L. angustifolia (2.1 mg/mL). Based on the obtained results, it is possible to conclude that tea tree essential oil might be used as an ecological antimicrobial in treating infectious diseases caused by the tested pathogens.