PDF(Pubmed)
Abstract：
Water bottles for everyday usage are a typical addition to people\'s life and offer a practical way to stay hydrated. Even though safe and clean water is preferred for consumption, the water bottle or container used to drink water was never considered to be cleaned. Here, we examined the adhering microbial populations in water bottles composed of stainless steel (SS) and polyethylene terephthalate (PET). A total of 30 water bottles-15 PET and 15 SS-were gathered from different users. To identify and quantify the adhering microbial populations, microbial swabs from the inside surface of the bottles were collected and later cultivated on certain growth media. Overall the microbial load of PET is significantly higher than the SS water bottles of 68.8 + 19.1 cfu/ml and 35.4 + 8 cfu/ml respectively at initial sampling (P = 0.0027). We also evaluated the efficiency of various cleaning procedures in eliminating adherent bacteria populations. The cleaning strategy significantly reduced the microbial load (P<0.0001). The mean load observed was 11.2 + 2.3 cfu/ml post-wash. This comparison study offers important new information about the adherent microbial populations found in SS and PET water bottles used every day, in the end. This finding emphasizes the necessity of routine cleaning and upkeep of these bottles to reduce the possibility of microbial contamination and the accompanying health risks.
摘要：
日常使用的水瓶是人们生活的典型补充，并提供了一种保持水分的实用方法。即使安全和干净的水是首选消费，用来喝水的水瓶或容器从未被认为是清洁的。这里,我们检查了由不锈钢（SS）和聚对苯二甲酸乙二醇酯（PET）组成的水瓶中粘附的微生物种群。从不同的用户收集了总共30个水瓶-15个PET和15个SS。为了识别和量化粘附的微生物种群，收集来自瓶子内表面的微生物拭子，然后在某些生长培养基上培养。总体上,PET的微生物负荷显著高于初始采样时分别为68.8+19.1cfu/ml和35.4+8cfu/ml的SS水瓶(P=0.0027)。我们还评估了各种清洁程序在消除粘附细菌种群中的效率。清洁策略显著降低了微生物负荷(P<0.0001)。观察到的平均负荷为11.2±2.3cfu/ml洗涤后。这项比较研究提供了有关每天使用的SS和PET水瓶中发现的粘附微生物种群的重要新信息，在最后。这一发现强调了对这些瓶子进行常规清洁和保养的必要性，以减少微生物污染的可能性和随之而来的健康风险。
