Metal Nanoparticles

金属纳米颗粒
  • 文章类型: Journal Article
    本文的目的是研究金属纳米颗粒在延长水果保质期中的应用。尽管人们对纳米粒子及其潜在应用的兴趣日益浓厚,目前很少有有效的方法来延长水果的保质期。这项研究集中在金属纳米颗粒延长保质期的基本原则上,包括氧化铜,氧化锌,银,和氧化钛。纳米粒子的生物学特性,尤其是那些具有抗菌特性的人,作为可能的水果保存解决方案引起了人们的兴趣。许多传统的保存方法都有缺点,包括昂贵的生产成本,保质期短,不良残留物,以及无法在自然环境中适当保存易腐水果。基于纳米技术的延长保质期的技术有可能解决这些问题。审查的重点是有效利用环境友好,绿色合成-生产的纳米颗粒,以延长水果的保质期。建立了这些纳米颗粒成功保存新鲜水果的能力。结果表明,通过使用纳米颗粒合成技术保存水果可能是一个可行的策略,为传统程序提供更有效和可持续的替代品。
    The purpose of this review was to investigate the application of metal nanoparticles in fruit shelf life extension. Despite growing interest in nanoparticles and their potential applications, there are currently few effective methods for prolonging the shelf life of fruits. The study concentrated on the principles underlying the shelf life extension of metallic nanoparticles, including copper oxide, zinc oxide, silver, and titanium oxide. The biological properties of nanoparticles, especially those with antibacterial qualities, have drawn interest as possible fruit preservation solutions. Many conventional preservation methods have drawbacks, including expensive production costs, short shelf lives, undesirable residues, and the incapacity to properly keep perishable fruits in their natural environments. Techniques for extending shelf life based on nanotechnology have the potential to get around these problems. The review focused on the effective use of environmentally benign, green synthesis-produced nanoparticles to extend the fruit shelf life. The ability of these nanoparticles to successfully preserve fresh fruits was established. The results imply that fruit preservation by the use of nanoparticle synthesis techniques may be a viable strategy, offering a more effective and sustainable substitute for traditional procedures.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    银纳米粒子(AgNPs),由于它们不寻常的特点,已被用于各种制药,化妆品,和保健产品。AgNPs,凭借其非凡的生物潜力,表现出抗菌,抗真菌药,抗病毒,抗炎,抗癌,和伤口愈合特性,并已广泛用于烧伤治疗。一些研究已经确定了银纳米颗粒在烧伤治疗中的用途,导致炎症减少,快速组织再生,以及胶原纤维的非凡创造。传统的物理和化学技术已经合成了AgNPs,但是它们看起来非常昂贵和危险。最近,由于其巨大的好处,人们对使用绿色化学方法合成AgNPs产生了相当大的兴趣,包括无毒,低能耗,无污染,经济,环保,更可持续。这篇综述强调了使用细菌绿色合成AgNPs,真菌,植物,和其他微生物以及目前有关绿色合成AgNPs在烧伤治疗中应用的研究,包括AgNPs的生物学方面,他们的行动模式,以及任何可能的有害影响。
    Silver nanoparticles (AgNPs), owing to their unusual characteristics, have been used in various pharmaceutical, cosmetic, and healthcare products. AgNPs, with their exceptional biological potential, exhibit antibacterial, antifungal, antiviral, anti-inflammatory, anticancer, and wound healing properties and have been extensively used in burn therapy. Several studies have established the use of silver nanoparticles in the treatment of burn injuries, resulting in reduced inflammation, quick tissue regeneration, and the remarkable creation of collagen fibers. Conventional physical and chemical techniques have synthesized AgNPs, but they appear to be highly costly and hazardous. Recently, there has been considerable interest in the synthesis of AgNPs using the green chemistry approach because of its tremendous benefits, including being non-toxic, low energy consumption, pollution-free, economical, environmentally friendly, and more sustainable. This review emphasizes the green synthesis of AgNPs using bacteria, fungi, plants, and other microorganisms and the current research related to the application of green synthesized AgNPs in burn therapy, including the biological aspects of AgNPs, their mode of action, and any possible detrimental effects.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Journal Article
    环保纳米技术启用的生物聚合物是包装材料替代传统合成聚合物及其复合材料的新概念之一。本文简明扼要地回顾了使用金属和金属氧化物纳米颗粒向生物聚合物引入额外官能团的最新进展。金属纳米粒子如银的功能,氧化锌,二氧化钛,氧化铜,黄金,还有氧化镁,作为食品包装材料进行了探讨。在生物聚合物中添加纳米颗粒可以改善机械性能,气体阻隔性能,耐用性,温度稳定性,水分稳定性,抗菌活性,抗氧化性能,和紫外线吸收,可以防止乙烯和氧气的存在,从而延长了食品的保质期。除此之外,这些生物聚合物复合膜的功能活性有助于它们像智能或智能包装一样起作用。金属纳米粒子的选择,粒子迁移,毒理学效应,并审查了食品包装行业的潜在未来范围。
    Eco-friendly nanotechnology-enabled biopolymers are one of the novel concepts of packaging materials to substitute traditional synthetic polymers and their composites. This article succinctly reviews the recent developments of introducing additional functionalities to biopolymers using metal and metal oxide nanoparticles. The functionality of metal nanoparticles such as silver, zinc oxide, titanium dioxide, copper oxide, gold, and magnesium oxide, as food packaging materials were discussed. The addition of nanoparticles in biopolymers improves mechanical properties, gas barrier properties, durability, temperature stability, moisture stability, antimicrobial activity, antioxidant property, and UV absorbance and can prevent the presence of ethylene and oxygen, hence extending the shelf life of foodstuffs. Other than this, the functional activity of these biopolymer composite films helps them to act like smart or intelligent packaging. The selection of metal nanoparticles, particle migration, toxicological effect, and potential future scope in the food packaging industry are also reviewed.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Journal Article
    本研究遵循PRISMA报告指南来呈现结果。对PubMed等电子数据库进行了全面搜索,Scopus,WebofSciences,科学直接。最初,检索到413篇文章。删除重复项并应用特定的纳入和排除标准后,本系统综述最终纳入10篇文章。
    综述的研究表明,硒纳米颗粒具有抗炎和抗氧化特性。它们有效地保护了肾脏,肝脏,和睾丸受损。此外,有证据表明所检查的器官具有有效的放射防护作用,没有明显的副作用。
    本系统综述强调了使用硒纳米颗粒防止电离辐射的负面影响的潜在优势。重要的是,这些保护作用是在没有引起明显副作用的情况下实现的。这些发现表明硒纳米颗粒作为辐射防护剂的潜在作用,提供可能的治疗应用,以减少医学影像和放射治疗程序中与电离辐射暴露相关的风险。
    UNASSIGNED: This study followed the PRISMA reporting guidelines to present the results. A comprehensive search was performed on electronic databases such as PubMed, Scopus, Web of Sciences, and Science Direct. Initially, 413 articles were retrieved. After removing duplicates and applying specific inclusion and exclusion criteria, 10 articles were finally included in this systematic review.
    UNASSIGNED: The reviewed studies showed that selenium nanoparticles had anti-inflammatory and antioxidant properties. They effectively protected the kidneys, liver, and testicles from damage. Furthermore, there was evidence of efficient radioprotection for the organs examined without significant side effects.
    UNASSIGNED: This systematic review emphasizes the potential advantages of using selenium nanoparticles to prevent the negative effects of ionizing radiation. Importantly, these protective effects were achieved without causing noticeable side effects. These findings suggest the potential role of selenium nanoparticles as radioprotective agents, offering possible therapeutic applications to reduce the risks related to ionizing radiation exposure in medical imaging and radiotherapy procedures.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    由于细菌病原体及其毒素引起的食源性疾病暴发已成为全球公共卫生和安全的严重关切。寻找具有针对当前腐败和食源性细菌病原体的独特作用机制的新型抗菌剂是克服抗生素抗性的中心策略。这项研究检查了无机纳米颗粒(NPs)对食源性细菌病原体的抗菌活性和作用机理。用英语写的文章是从寄存器和数据库中恢复的(PubMed,ScienceDirect,WebofScience,谷歌学者,和开放存取期刊目录)和其他来源(网站,组织,和引文搜索)。\"纳米粒子,\"\"无机纳米粒子,\"\"金属纳米颗粒,\"\"金属氧化物纳米颗粒,\“\”抗菌活性,\"\"抗菌活性,\"\"食源性细菌病原体,“\”行动机制,“”和“食源性疾病”是用于检索文章的搜索词。PRISMA-2020清单适用于文章搜索策略,文章选择,数据提取,和审查过程的结果报告。从不同的搜索策略获得的3,575篇文章中,共包括27篇原始研究文章。所有研究都证明了无机NPs的抗菌效果,并强调了它们对食源性细菌病原体的不同作用机制。在本研究中,小尺寸,球形,工程,封顶,与水的低溶解,高浓度NP,在革兰氏阴性细菌类型中,与它们的对应物相比具有很高的抗菌活性。细胞壁相互作用和膜渗透,活性氧的产生,DNA损伤,和蛋白质合成抑制是当前研究中公认的一些广义机制。因此,这项研究建议在食品加工行业中正确使用无毒的无机纳米颗粒产品,以确保食品的质量和安全,同时最大限度地减少食源性细菌病原体对抗生素的耐药性。
    Foodborne disease outbreaks due to bacterial pathogens and their toxins have become a serious concern for global public health and security. Finding novel antibacterial agents with unique mechanisms of action against the current spoilage and foodborne bacterial pathogens is a central strategy to overcome antibiotic resistance. This study examined the antibacterial activities and mechanisms of action of inorganic nanoparticles (NPs) against foodborne bacterial pathogens. The articles written in English were recovered from registers and databases (PubMed, ScienceDirect, Web of Science, Google Scholar, and Directory of Open Access Journals) and other sources (websites, organizations, and citation searching). \"Nanoparticles,\" \"Inorganic Nanoparticles,\" \"Metal Nanoparticles,\" \"Metal-Oxide Nanoparticles,\" \"Antimicrobial Activity,\" \"Antibacterial Activity,\" \"Foodborne Bacterial Pathogens,\" \"Mechanisms of Action,\" and \"Foodborne Diseases\" were the search terms used to retrieve the articles. The PRISMA-2020 checklist was applied for the article search strategy, article selection, data extraction, and result reporting for the review process. A total of 27 original research articles were included from a total of 3,575 articles obtained from the different search strategies. All studies demonstrated the antibacterial effectiveness of inorganic NPs and highlighted their different mechanisms of action against foodborne bacterial pathogens. In the present study, small-sized, spherical-shaped, engineered, capped, low-dissolution with water, high-concentration NPs, and in Gram-negative bacterial types had high antibacterial activity as compared to their counterparts. Cell wall interaction and membrane penetration, reactive oxygen species production, DNA damage, and protein synthesis inhibition were some of the generalized mechanisms recognized in the current study. Therefore, this study recommends the proper use of nontoxic inorganic nanoparticle products for food processing industries to ensure the quality and safety of food while minimizing antibiotic resistance among foodborne bacterial pathogens.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    具有不同形貌的新型纳米颗粒的生物合成,这对他们的生物能力有很好的影响,在纳米技术领域引起了越来越多的关注。真菌提取物中存在的生物活性化合物,细菌,植物和藻类负责纳米粒子的合成。与其他生物资源相比,棕色海藻也可用于将金属离子转化为金属纳米颗粒,因为存在更丰富的生物活性化学物质。碳水化合物,蛋白质,多糖,维生素,酶,颜料,棕色海藻中的次生代谢产物起到自然还原的作用,capping,和纳米粒子合成中的稳定剂。大约有2000种海藻主导着海洋资源,但只有少数报道了纳米粒子的合成。生物活性化学物质在生物合成的金属纳米颗粒中的存在赋予生物活性。褐藻生物合成的金属和非金属纳米颗粒因其不同的理化性质而具有不同的生物活性。与陆地资源相比,海洋资源对纳米粒子合成的探索并不多。为了确认它们的形态,使用表征方法,如吸收分光光度计,X射线衍射,傅里叶变换红外光谱,扫描电子显微镜,和透射电子显微镜。这篇综述试图包括褐藻在金属和非金属纳米粒子合成中的重要作用,以及合成方法和生物应用,如抗癌,抗菌,抗氧化剂,抗糖尿病,和其他功能。
    The biosynthesis of novel nanoparticles with varied morphologies, which has good implications for their biological capabilities, has attracted increasing attention in the field of nanotechnology. Bioactive compounds present in the extract of fungi, bacteria, plants and algae are responsible for nanoparticle synthesis. In comparison to other biological resources, brown seaweeds can also be useful to convert metal ions to metal nanoparticles because of the presence of richer bioactive chemicals. Carbohydrates, proteins, polysaccharides, vitamins, enzymes, pigments, and secondary metabolites in brown seaweeds act as natural reducing, capping, and stabilizing agents in the nanoparticle\'s synthesis. There are around 2000 species of seaweed that dominate marine resources, but only a few have been reported for nanoparticle synthesis. The presence of bioactive chemicals in the biosynthesized metal nanoparticles confers biological activity. The biosynthesized metal and non-metal nanoparticles from brown seaweeds possess different biological activities because of their different physiochemical properties. Compared with terrestrial resources, marine resources are not much explored for nanoparticle synthesis. To confirm their morphology, characterization methods are used, such as absorption spectrophotometer, X-ray diffraction, Fourier transforms infrared spectroscopy, scanning electron microscope, and transmission electron microscopy. This review attempts to include the vital role of brown seaweed in the synthesis of metal and non-metal nanoparticles, as well as the method of synthesis and biological applications such as anticancer, antibacterial, antioxidant, anti-diabetic, and other functions.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Journal Article
    寻常痤疮(AV),皮脂腺中的皮脂产生过多和痤疮杆菌增殖,显着影响身心健康。最近的治疗进展集中在皮脂腺的选择性光热解。这篇综述评估了两种创新疗法:1726nm激光和纳米粒子辅助激光治疗。我们使用主要术语“寻常痤疮”或“痤疮”和“激光”对PubMed和Embase进行了全面搜索,\"\"光热疗法,\"\"纳米粒子,\"\"治疗,“或”1726nm激光。“纳入标准是在同行评审的期刊上以英文发表的文章,重点是通过针对皮脂腺来治疗AV,产生11项研究。金纳米粒子,与800纳米激光一起使用,1064-nmNd:YAG激光器,或光气动装置,和铂纳米粒子与1450纳米二极管激光,显示痤疮病变的严重程度和数量显着改善,安全,患者满意度。1726nm激光治疗也显示出相当大的损伤减少和耐受性,具有最小的副作用,如红斑和水肿。它的效率归功于它的简短,有效靶向皮脂腺的高功率脉冲,提供精确的治疗与更少的副作用相比,低功率脉冲。使用纳米粒子辅助激光治疗或1726nm激光的选择性光热分解为传统AV治疗提供了有希望的替代方案,展示疗效和高患者满意度。1726纳米激光流线治疗,但涉及新的设备成本,虽然纳米粒子辅助疗法很好地整合到现有的设置中,但依赖于外部药物,不适合某些过敏。未来的研究应包括长期研究和比较分析。治疗方式的选择应考虑患者的喜好,成本影响,以及特定疗法的可用性。
    Acne vulgaris (AV), characterized by excessive sebum production and Cutibacterium acnes proliferation in the sebaceous glands, significantly impacts physical and psychological health. Recent treatment advancements have focused on selective photothermolysis of sebaceous glands. This review evaluates two innovative therapies: the 1726-nm laser and nanoparticle-assisted laser treatments. We conducted a comprehensive search of PubMed and Embase using the primary terms \"acne vulgaris\" or \"acne\" AND \"laser,\" \"photothermal therapy,\" \"nanoparticles,\" \"treatment,\" or \"1726 nm laser.\" Inclusion criteria were articles published in English in peer-reviewed journals that focused on treating AV through targeting the sebaceous glands, yielding 11 studies. Gold nanoparticles, used with 800-nm laser, 1064-nm Nd: YAG laser, or photopneumatic device, and platinum nanoparticles with 1450-nm diode laser, showed notable improvements in severity and number of acne lesions, safety, and patient satisfaction. The 1726-nm laser treatments also showed considerable lesion reduction and tolerability, with minimal side effects such as erythema and edema. Its efficiency is credited to its short, high-power pulses that effectively target sebaceous glands, offering precise treatment with fewer side effects compared to lower-power pulses. Selective photothermolysis using nanoparticle-assisted laser therapy or the 1726-nm laser offers a promising alternative to conventional AV treatments, showcasing efficacy and high patient satisfaction. The 1726-nm laser streamlines treatment but involves new equipment costs, while nanoparticle-assisted therapy integrates well into existing setups but relies on external agents and is unsuitable for certain allergies. Future research should include long-term studies and comparative analyses. The choice of treatment modality should consider patient preferences, cost implications, and availability of specific therapies.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Systematic Review
    正畸治疗,虽然对于实现最佳口腔健康至关重要,由于正畸矫治器上细菌粘附和生物膜形成的倾向,在感染控制方面面临挑战。镀银正畸材料已经成为一种有前途的解决方案,利用银纳米颗粒(AgNPs)的有效抗菌性能。在正畸中使用抗菌涂层来防止细菌生物膜的形成。这篇系统的综述评估了固定正畸矫治器上抗菌银涂层的文献,包括弓丝,括号,和微植入物。两名评估人员,独立工作,严格对各种数据库进行全面搜索,包括PubMed,PubMedCentral,Embase,Scopus和WebofScience本系统综述全面检查了体外研究,研究了镀银正畸弓丝的抗菌功效,括号,和微植入物。PROSPEROCRD42024509189中注册的评论综合了18项不同研究的结果,揭示细菌粘附的一致和显著减少,生物膜的形成,和菌落计数与AgNP的掺入。关键研究证明了银涂层弓丝和托槽对常见口腔细菌的有效性,如变形链球菌和金黄色葡萄球菌。涂覆有AgNP的微植入物还表现出对一系列微生物的显著抗微生物活性。系统评价揭示了这些抗菌作用的潜在机制,强调了正畸实践中预防感染的意义,并提出了未来的研究途径。尽管有一些研究的异质性和局限性,集体证据支持镀银正畸材料在减轻细菌并发症方面的潜力,强调它们在推进正畸感染控制措施中的相关性。
    Orthodontic treatments, while essential for achieving optimal oral health, present challenges in infection control due to the propensity for bacterial adhesion and biofilm formation on orthodontic appliances. Silver-coated orthodontic materials have emerged as a promising solution, leveraging the potent antimicrobial properties of silver nanoparticles (AgNPs). Antibacterial coatings are used in orthodontics to prevent the formation of bacterial biofilms. This systematic review evaluated the literature on antimicrobial silver coatings on fixed orthodontic appliances, including archwires, brackets, and microimplants. Two evaluators, working independently, rigorously conducted a comprehensive search of various databases, including PubMed, PubMed Central, Embase, Scopus and Web of Science. This systematic review comprehensively examined in vitro studies investigating the antimicrobial efficacy of silver-coated orthodontic archwires, brackets, and microimplants. The review registered in PROSPERO CRD42024509189 synthesized findings from 18 diverse studies, revealing consistent and significant reductions in bacterial adhesion, biofilm formation, and colony counts with the incorporation of AgNPs. Key studies demonstrated the effectiveness of silver-coated archwires and brackets against common oral bacteria, such as Streptococcus mutans and Staphylococcus aureus. Microimplants coated with AgNPs also exhibited notable antimicrobial activity against a range of microorganisms. The systematic review revealed potential mechanisms underlying these antimicrobial effects, highlighted implications for infection prevention in orthodontic practice, and suggested future research avenues. Despite some study heterogeneity and limitations, the collective evidence supports the potential of silver-coated orthodontic materials in mitigating bacterial complications, emphasizing their relevance in advancing infection control measures in orthodontics.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    双金属纳米粒子(BMNPs)因其显著的催化性能而受到广泛关注,使它们在废水处理应用中无价。这些挑战之一在于BMNPs由于范德华相互作用而聚集的倾向,这会降低它们的整体性能。此外,从处理过的溶液中检索耗尽的NP用于后续重复使用仍然是一个重大障碍。此外,NPs浸出到排放的废水中会对人类和水生生物产生有害影响。为了克服这些问题,已经研究了各种底物以最大化NP的效率和稳定性。这篇综述论文探讨了各种底物在固定化BMNPs中的关键作用,对他们的表现进行全面分析,优势,和缺点。基材包括各种各样的材料,包括有机的,无机,有机-无机,珠子,纤维,和膜。每种基材类型提供独特的属性,影响稳定性,效率,和BMNPs的可回收性。这篇综述论文旨在提供用于BMNPs固定化的底物的最新和详细的分析和比较。这项工作进一步回顾了处理废水污染物的复合材料的潜在机制,以及这些机制如何通过底物和BMNPs产生的协同效应得到增强。此外,讨论了这些复合材料的可重用性和可持续性。此外,高性能底物被强调为未来的研究方向,重点是BMNPs的固定化在废水处理中的应用。
    Bimetallic nanoparticles (BMNPs) have gained considerable attention due to their remarkable catalytic properties, making them invaluable in wastewater treatment applications. One of these challenges lies in the propensity of BMNPs to aggregate due to Van der Waals interactions, which can reduce their overall performance. Additionally, retrieving exhausted NPs from the treated solution for subsequent reuse remains a significant hurdle. Moreover, the leaching of NPs into the discharged wastewater can have harmful effects on humans as well as aquatic life. To overcome these issues, various substrates have been researched to maximize the efficiency and stability of the NPs. This review paper delves into the pivotal role of various substrates in immobilizing BMNPs, providing a comprehensive analysis of their performances, advantages, and drawbacks. The substrates encompass a diverse range of materials, including organic, inorganic, organic-inorganic, beads, fibers, and membranes. Each substrate type offers unique attributes, influencing the stability, efficiency, and recyclability of BMNPs. This review paper aims to provide an up-to-date and detailed analysis and comparison of the substrates used for the immobilization of BMNPs. This work further reviews the underlying mechanisms of the composites involved in treating pollutants from wastewater and how these mechanisms are enhanced by the synergistic effects produced by the substrate and BMNPs. Furthermore, the reusability and sustainability of these composites are discussed. Also, high-performing substrates are highlighted to give direction to future research focusing on the immobilization of BMNPs in the application of wastewater treatment.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Journal Article
    如今,使用生物方法合成纳米颗粒作为使用高能耗和昂贵和危险材料消耗的方法的替代品是全世界研究人员感兴趣的。合成金属纳米颗粒的生物方法非常重要,因为它们很容易,负担得起的,安全,环保,能够控制纳米粒子的大小和形状。当今感兴趣的方法之一是在金属纳米颗粒的合成中使用噬菌体作为自然界中最丰富的生物体。从噬菌体生物合成的纳米材料已经显示出各种临床应用,包括抗菌活性,生物医学传感器,药物和基因传递系统,癌症治疗和组织再生。因此,这篇综述的目的是研究带有噬菌体的金属纳米颗粒的生物合成及其生物医学应用。
    Nowadays, the use of biological methods of synthesis of nanoparticles as substitutes for methods that use high energy and consumption of expensive and dangerous materials is of interest to researchers all over the world. Biological methods of synthesising metal nanoparticles are very important because they are easy, affordable, safe, environmentally friendly and able to control the size and shape of nanoparticles. One of the methods that is of interest today is the use of bacteriophages as the most abundant organisms in nature in the synthesis of metal nanoparticles. Nanomaterials biosynthesized from phages have shown various clinical applications, including antimicrobial activities, biomedical sensors, drug and gene delivery systems, cancer treatment and tissue regeneration. Therefore, the purpose of this review is to investigate the biosynthesis of metal nanoparticles with phages and their biomedical applications.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

公众号