慢性子宫内膜炎是子宫粘膜内层的炎症,不管有没有感染过程,这会影响动物的生育能力,但不会影响其总体健康。多年来已经采用了多种治疗方法,但迄今为止,没有有效的治疗方法能够更新受伤的组织。由于胎母交流的缺陷是由慢性子宫内膜炎症引起的退行性变化引起的,我们的工作假设是再生医学利用羊膜来源的微囊泡(MVs)的抗炎和再生作用来治疗这种疾病的新方法.MV负责遗传物质的水平转移,包括参与原始细胞和靶细胞之间的旁分泌通信的microRNA(miRNA)。因此,宫内输注MV可能对退行性慢性子宫内膜炎和胎儿-母体谈话有益。被选中的母马是11岁的弗利西亚,尽管多次尝试授精,但仍有失败的怀孕史。准时和明显的热是生殖史的特征,但是多年来没有进行过授精尝试。第一次(失败的)授精是在母马9岁时。在接下来的两个繁殖季节,定期进行其他尝试,但均未成功。在使用经过证实的生育能力的种马进行最后的授精尝试之后,对一个8日龄胚胎的采集表明,该母马受到与子宫内膜炎相关的着床失败的影响.对母马进行了两个周期的羊膜源MV的子宫内给药。子宫内膜炎的分类和成功的人工授精(AI)与胚胎植入的改善证明了子宫内膜给药的成功。在第14天检测到,并且怀孕仍在进行中。可能,MVs能够恢复受损的子宫内膜,并重新建立正确的沟通,以成功植入胚胎。
Chronic endometritis is an inflammation in the inner layer of uterine mucosa, with or without an infectious process, which affects the animal\'s fertility but not its general health. A variety of treatments has been adopted over the years but to date, no effective cures have been able to renew the injured tissue. Since the defects in the fetal-maternal communication are caused by degenerative changes due to chronic endometrial inflammation, our working hypothesis was a new approach to this disease by the regenerative medicine using amniotic derived microvesicles (MVs) for their anti-inflammatory and regenerative effects. The MVs are responsible for horizontal transfer of genetic materials, including microRNA (miRNAs) that are involved in paracrine communication between origin cells and target cells. Thus, intrauterine MV infusion may be beneficial in degenerative chronic endometritis and in the fetal-maternal talk. The selected
mare was an 11-year-old Friesian, with a history of failed pregnancies despite numerous insemination attempts. Punctual and evident heats characterized the reproductive history, but no insemination attempts had been made for many years. The first (failed) insemination was when the
mare was 9-years-old. In the next two reproductive seasons, other attempts were made at regular intervals but none was successful. After a final insemination attempt using a stallion of proven fertility, the collection of an 8-day old embryo suggested that the
mare was affected by implantation failure related to endometritis. The
mare was treated with two cycles of intrauterine administration of amniotic-derived MVs. The success of the intrauterine administration of MVs was demonstrated by an improvement in the classification of endometritis and in a successful artificial insemination (AI) with implantation of an embryo, as detected at day 14 and with a pregnancy that is still ongoing. Probably, MVs were able to restore the injured endometrium and re-establish the proper communication for a successful embryo implantation.