背景:巨噬细胞抑制因子(MIF)是由几种细胞分泌的促炎细胞因子,包括免疫系统和皮肤.MIF基因在能够影响其活性的启动子区域中含有SNP-173G>C和STR-794CATT5-8多态性。我们的目的是调查MIF多态性作为墨西哥人群斑块状银屑病(PP)的危险因素。
方法:我们通过PCR-RFLP方法对224例具有临床和组织病理学诊断的PP患者和232例对照受试者(CS)的MIF多态性(rs5844572和rs755622)进行了基因分型。通过ELISA试剂盒测定MIF血清水平。
结果:我们发现MIF-173G>C多态性的基因型和等位基因频率存在显着差异;GC基因型(OR1.51,95%CI1.026-2.228,p=0.03)和C等位基因(OR1.34,95%CI1.005-1.807,p=0.04)的携带者出现PP的几率更高。此外,6C单倍型与PP风险相关(OR2.10,95%CI1.22-3.69,p<0.01)。此外,-173CC基因型与高MIF血清水平相关(p<0.05)。
结论:MIF多态性的-173GC基因型和6C单倍型与墨西哥人群对PP的易感性相关。
BACKGROUND: Macrophage inhibitory factor (
MIF) is a pro-inflammatory cytokine secreted by several cells, including those in the immune system and the skin. The
MIF gene contains the SNP -173 G> C and STR -794 CATT5-8 polymorphisms in the promoter region capable of affecting its activity. Our objective was to investigate the
MIF polymorphisms as a risk factor for plaque psoriasis (PP) in the Mexican population.
METHODS: We genotyped both MIF polymorphism (rs5844572 and rs755622) in 224 PP patients with a clinical and histopathological diagnosis and 232 control subjects (CS) by the PCR-RFLP method.
MIF serum levels were determined by an ELISA kit.
RESULTS: We found significant differences in the genotypic and allelic frequencies for the MIF -173 G>C polymorphism; carriers of the GC genotype (OR 1.51, 95% CI 1.026-2.228, p = 0.03) and the C allele (OR 1.34, 95% CI 1.005-1.807, p = 0.04) had higher odds to present with PP. Moreover, the 6C haplotype was associated with PP risk (OR 2.10, 95% CI 1.22-3.69, p < 0.01). Also, the -173 CC genotype was associated with high
MIF serum levels (p < 0.05).
CONCLUSIONS: The -173 GC genotype and the 6C haplotype of the MIF polymorphisms are associated with susceptibility to PP in the Mexican population.