蓝细菌是一类古老的光自养原核生物，并在全球碳循环中发挥重要作用。它们也是研究光合作用和昼夜节律调节的模式生物，代谢工程和合成生物学策略授予光驱动生物技术应用于蓝细菌，特别是用于工程蓝藻细胞，以实现有效的光驱动系统，用于从可再生原料中合成任何感兴趣的产物。然而,较低的产量限制了蓝藻合成生物学的工业应用潜力，和一些关键的限制必须克服，以实现这些多功能微生物的全部生物技术潜力。尽管针对蓝藻的基因工程工具包取得了一些进展，现有的工具仍然落后于传统的异养微生物。因此,本研究描述了蓝藻基因工程的现状和局限性，并提出进一步改进，以提高目标产品的产量。我们相信，蓝藻介导的光驱动平台，以高效合成绿色化学品，可以通过开发用于菌株操纵的工具和具有出色生物技术应用性能的新型底盘生物来解锁光明的未来。这也可以加速生物制造业的发展。
Cyanobacteria are an ancient group of photoautotrophic prokaryotes, and play an essential role in the global carbon cycle. They are also model organisms for studying photosynthesis and circadian regulation, and metabolic engineering and synthetic biology strategies grants light-driven biotechnological applications to cyanobacteria, especially for engineering cyanobacteria cells to achieve an efficient light-driven system for synthesizing any product of interest from renewable feedstocks. However, lower yield limits the potential of industrial application of cyanobacterial synthetic biology, and some key limitations must be overcome to realize the full biotechnological potential of these versatile microorganisms. Although genetic engineering toolkits for cyanobacteria have made some progress, the tools available still lag behind conventional heterotrophic microorganism. Consequently, this study describes the current situations and limitations of genetic engineering in cyanobacteria, and further improvements are proposed to improve the output of targeted products. We believe that cyanobacteria-mediated light-driven platforms towards efficient synthesis of green chemicals could unlock a bright future by developing the tools for strain manipulation and novel chassis organisms with excellent performance for biotechnological applications, which could also accelerate the advancement of bio-manufacturing industries.
